PHASE BEHAVIOR OF FISH GELATIN–AGAR AQUEOUS MIXTURES

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Abstract

The interaction of fish gelatin and agar in the bulk of the aqueous phase with the formation of supramolecular protein–polysaccharide complexes was studied using spectroscopy (IR Fourier, UV), turbidimetry, quasi-elastic laser light scattering and scanning electron microscopy. The influence of the structure of gelatin (content of amino acid residues), the agar/fish gelatin w/w ratio Z and the medium (pH, ionic strength I) on the boundaries of the regions of stoichiometric and non-stoichiometric complexes formation, the size and ζ-potential of particles and, consequently, the phase behavior of the aqueous mixture (i.e., colloidal solution) of biopolymers was considered. Phase diagrams of aqueous mixtures of fish gelatin and agar were constructed in the coordinates Z – characteristic pH, as well as I – characteristic pH. The regions of different phase behavior of the systems are determined, such as a single-phase solution of non-complexed biopolymers, a dispersion of fish gelatin–agar complexes, the region of the beginning of the separation of coacerates from the dispersion, and the region of complete separation of the coacervate phase and supernatant. It is shown that the formation of fish gelatin–agar complexes affects the microstructure of gels formed during cooling of aqueous mixtures of biopolymers.

About the authors

N. G. Voron’ko

Murmansk Arctic University

Email: voronkong@mauniver.ru
Murmansk, Russia

T. D. Kuzina

Murmansk Arctic University

Email: email@example.com
Murmansk, Russia

D. S. Kolotova

Murmansk Arctic University

Email: email@example.com
Murmansk, Russia

Yu. A. Kuchina

Murmansk Arctic University

Email: email@example.com
Murmansk, Russia

Yu. F. Zuev

Kazan Institute of Biochemistry and Biophysics, Federal Research Center "Kazan Scientific Center", Russian Academy of Sciences

Email: email@example.com
Kazan, Russia

S. R. Derkach

Murmansk Arctic University

Email: email@example.com
Murmansk, Russia

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