Targeted Nanoliposomes Loaded with IR780 Dye as a Multifunctional Nanoplatform for Photothermal and Photodynamic Cancer Therapy

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Abstract

During cancer treatment the combined effect of photothermal and photodynamic therapy has unique advantages over each of these methods alone. In this study, a multifunctional targeted nanoplatform for simultaneous combined photothermal and photodynamic therapy under 808 nm infrared laser irradiation was developed. The developed system consists of ~140 nm liposomes specific to the tumor-associated HER2 antigen and loaded with the near-infrared heptamethincyanine dye IR780. The targeting of liposomes to the HER2 is determined by the HER2-specific scaffold protein DARPin_9-29 located on the outer surface of liposomes. It has been established that IR-780, loaded in liposomes, retains photothermal and photodynamic properties: upon irradiation, the temperature of IR780-loaded liposome solution rapidly increases (up to 60°C within 60 s), and the production of reactive oxygen species is also detected. In vitro experiments have shown that HER2-specific liposomes containing IR780 have photoinduced cytotoxicity against HER2-overexpressing cells, causing the death of 50% of the cell population at a concentration of 2.85 μM. The results of the study suggest that HER2-specific liposomes containing IR780 have excellent targeted characteristics, and IR780 can be used as an active substance for simultaneous photothermal and photodynamic therapy.

About the authors

G. M Proshkina

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, RAS

Email: gmb@ibch.ru
Moscow, Russia

E. I Shramova

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, RAS

Moscow, Russia

A. S Sogomonyan

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, RAS; Moscow Institute of Engineering Physics, National Research Nuclear University "MEPhI"

Moscow, Russia; Moscow, Russia

S. M Deyev

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, RAS; Moscow Institute of Engineering Physics, National Research Nuclear University "MEPhI"; Ogarev National Research Mordovian State University

Moscow, Russia; Moscow, Russia; Saransk, Russia

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