The Effect of Gamma Radiation on the Mitotic Index and Root Growth Endpoints of Allium Cepa Seedlings in Long-Term Experiments

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Abstract

The effect of γ-radiation including low doses on the mitotic index and root growth endpoints of Allium cepa (L.) seedlings in different intervals of the recovery time after irradiation were studied. Onion seedlings were irradiated with 137Cs point γ-sources of various activity, including radioactive microparticles of the Yenisei River floodplain. According to the results of 7 experiments, the inhibitory effect of γ-irradiation on onion root growth was observed in the dose range from 0.1 to 21 Gy in the time interval of the recovery period of up to 6 days, by the 10th day the damaging effect of γ-irradiation persisted mainly at absorbed doses of 5.2 Gy. A detailed examination of root growth inhibition in the time interval from 1 to 6 days of the experiment allowed us to determine that a sharp inhibition of root growth began on day 4 at absorbed doses of 2.5–10.7 Gy, and at the same time a decrease in proliferative activity in the apical meristem of the root was observed. The results of a factor analysis of the experiment on the dynamics of inhibition of primary root length and mitotic index after irradiation are presented.

About the authors

E. A Trofimova

Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS"

Email: e.trofimova11@yandex.ru
ORCID iD: 0000-0001-7511-4916
junior researcher, radioecology laboratory, Krasnoyarsk, Russia

A. Ya Bolsunovsky

Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS"

Email: radecol@ibp.ru
ResearcherId: P‑8028-2015
Dr. Sc. (Biology), head of radioecology laboratory Krasnoyarsk, Russia

D. V Dementyev

Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS"

Email: dementyev@gmail.com
ORCID iD: 0000-0002-7016-8592
senior researcher radioecology laboratory Krasnoyarsk, Russia

M. V Petrichenkov

Budker Institute of Nuclear Physics of the Siberian Branch of the RAS

Email: petrmv@gmail.com
ORCID iD: 0000-0002-4125-3798
is the head of the department for Radiation Research and Radiation Safety Novosibirsk, Russia

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