Immunological effect of moderately hypoxic mesenchymal stromal cell culture conditions on uterine healing in rats

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The aim is to determine the influence of conditioned medium of autologous bone marrow stromal cells (CMSC) cultured at 10% O2, on the healing of the rat uterus after a thorough surgical incision. Methods: In the treated group of animals (n = 14), the edges of the uterine wound were processed with a conditioned medium prior to suturing, in the control group of animals (n = 14), the incision was not processed. Results: On the 5th and 15th days after the operation, morphological studies of the healing zone were carried out. The number of macrophages CD68, FABP4+ cells and the level of mRNA expression of inflammatory cytokine IL-1b, IL-6 and anti-inflammatory IL-4, IL-10 were assessed. The healing area in the treated group was lower than the reference area in all monitoring periods (p < 0.05). From the 5th to the 15th day, the expression of IL-1b, IL-4, IL-6 and IL-10 in the control group decreased (p < 0.05), in the processed group no valid differences were found (p < 0.05). On the 5th day in the treatment group, the expression of IL-4 and IL-10 was lower than in the control, and on the 15th day the expression of IL-1b was lower in the control group. The number of macrophages of CD68 decreased by 15th day in the treated group compared to 5th day and was lower than in the control group. The FABP4 cell clusters in the area of damaged myometrium at 15 days in both groups were almost eliminated except for 2 animals in the control group. In the area of damaged perimetrium, FABP4 clusters increased in the control group by 15th day, and in the treated group decreased and was reliably lower than in the control group. Thus, the application of CMSC has resulted in a reduction of the damage area, reduced inflammatory response and accelerated recovery of the uterine wall.

作者简介

N. Tikhonova

Petrovsky National Research Center of Surgery

Email: morpholhum@list.ru
Москва, Россия

A. Temnov

Institute of Cell Biophysics of the Russian Academy of Sciences

Пущино, Россия

V. Aleksankina

Petrovsky National Research Center of Surgery

Москва, Россия

T. Fokina

Petrovsky National Research Center of Surgery

Москва, Россия

A. Aleksankin

Petrovsky National Research Center of Surgery

Москва, Россия

A. Sklifas

Institute of Cell Biophysics of the Russian Academy of Sciences

Пущино, Россия

E. Kuznetsova

Petrovsky National Research Center of Surgery

Москва, Россия

A. Milovanov

Petrovsky National Research Center of Surgery

Москва, Россия

L. Mikhaleva

Petrovsky National Research Center of Surgery

Москва, Россия

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