GynecologyGynecology2079-56962079-5831Consilium Medicum28645Research ArticlePregnancy and genital mycoplasma outcomesKarapetyanT Etomamed02@mail.ruV.I.Kulakov Research Center for Obstetrics, Gynecology and Perinatology of the Ministry of Health of the Russian Federation15062017193737609042020Copyright © 2017, Consilium Medicum2017The aim of the study was to determine the possible association of adverse pregnancy outcomes and genital mycoplasmas colonizing the vagina of women during pregnancy. Materials and methods. The results of a survey of 312 pregnant women from the group at increased risk of developing inflammatory complications were subjected to multifactorial analysis. The state of the vaginal biotope was determined on the basis of an integrated assessment of the results of the microscopy of vaginal grammy-smears and the sowing of the vaginal discharge to be facultative-anaerobic and microaerophilic groups of microorganisms. 5% of blood agar was used as the most universal medium, as well as Saburo agar (for selective isolation of fungi) and MRS agar (for isolating lactobacilli) to seed the vaginal discharge with a quantitative estimate of the growth of microorganisms. To isolate, identify, differentiate and quantify the growth of genital mycoplasmas, the Mycoplasma DUO test kits ("BioRad") were used. The analysis of clinical parameters - parameters of obstetric-gynecological history, current of pregnancy, childbirth, postpartum inflammatory complications and the state of newborns was carried out. Results. The analysis of the frequency of mycoplasma release depending on the state of the microcenosis of the vagina showed that the frequency of mycoplasma release was statistically significantly higher for bacterial vaginosis (74.6%) and its combination with candidal vaginitis (73.7%) compared with normocenosis (46, 8%, p<0.05, p>0.01). The conducted study allows to consider as inconsistent the examination of women with unfavorable outcomes of previous pregnancies to identify foci of chronic bacterial infections and / or antiphospholipid antibodies.bacterial vaginosispregnancymycoplasmaбактериальный вагинозбеременностьмикоплазмы[Cox C, Saxena N, Watt A.P et al. The common vaginal commensal bacterium Ureaplasmaparvum is associated with chorioamnionitis in extreme preterm labor. J Matern Fetal Neonatal Med 2016; 29 (22): 3646-51.][Белова А.В., Асцатурова О.Р., Александров Л.С. и др. Генитальные микоплазмы (Ureaplasma parvum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium) в развитии осложнений беременности, родов и послеродового периода. Архив акушерства и гинекологии им. В.Ф.Снегирева. 2014; 1 (2): 26-31.][Белова А.В., Никонов А.П. Генитальные микоплазмы (U. parvum, U. urealyticum, M. hominis, M. genitalium) в структуре инфекционных осложнений в акушерстве, гинекологии и перинатологии. Альманах клин. медицины. 2015; 39: 140-50.][Averbach S.H, Hacker M.R, Yiu T et al. Mycoplasma genitalium and preterm delivery at an urban community health center. Int J Gynaecol Obstet 2013; 123 (1): 54-7.][Dehghan Marvast L, Aflatoonian A, Talebi A.R et al. Relationship between Chlamydia trachomatis and Mycoplasma genitalium infection and pregnancy rate and outcome in Iranian infertile couples. Andrologia 2016; 29.][Otgonjargala B, Becker K, Batbaatar G et al. Effect of Mycoplasma hominis and cytomegalovirus infection on pregnancy outcome: A prospective study of 200 Mongolian women and their newborns. PLoS One 2017; 12 (3).][Анкирская А.С., Муравьева В.В. Интегральная оценка состояния микробиоты влагалища. Диагностика оппортунистических вагинитов (медицинская технология). М., 2011.][Ramazanzadeh R, Khodabandehloo M, Farhadifar F et al. A Case - control Study on the Relationship between Mycoplasma genitalium Infection in Women with Normal Pregnancy and Spontaneous Abortion using Polymerase Chain Reaction. Osong Public Health Res Perspect 2016; 7 (5): 334-8.][Rowlands S, Danielewski J.A, Tabrizi S.N et al. Microbial invasion of the amniotic cavity in midtrimester pregnancies using molecular microbiology. Am J Obstet Gynecol 2017. PII: S0002-9378(17)30383-6.][Donders G.G, Ruban K, Bellen G, Petricevic L. Mycoplasma/Ureaplasma infection in pregnancy: to screen or not to screen. J Perinat Med 2017; 18.][Kotb M. Bacterial pyrogenic exotoxins as superantigens. Clin Microbiol Rev 1995; 8: 411-26.][Choi S.J, Park S.D, Jang I.H et al. The prevalence of vaginal microorganisms in pregnant women with preterm labor and preterm birth. Ann Lab Med 2012; 32 (3): 194-200.]