GynecologyGynecology2079-56962079-5831Consilium Medicum30011Research ArticleDiagnostic significance of the determination of peripheral blood lymphocytes for predicting the onset of pregnancy in patients with repeated implant failures in groups with different protocols of the assisted reproductive technology programAmyanT Samyantanya@rambler.ruKrechetovaL Vk_l_v_@mail.ruPerminovaS Gperisvet@list.ruVtorushinaV Vvtorushina@inbox.ruSelimovaF Ndoc.fselimova@mail.ruV.I.Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology of the Ministry of Health of the Russian Federation15102017195262909042020Copyright © 2017, Consilium Medicum2017Purpose of the study. Study of the diagnostic significance of the determination of peripheral blood lymphocytes with phenotypes significantly different from the control values for predicting the onset of pregnancy in groups with different protocols of the assisted reproductive technology program in patients with repeated implant failures. Material and methods. The study included 70 patients with repeated failures of implantation in the in vitro fertilisation program: 33 patients in a protocol with a gonadotropin-releasing hormone antagonist (antHNRH), 37 patients in a cryocycle. Results of the study. Patients in the stimulated cycle showed a high diagnostic significance of the definition of a subpopulation of lymphocytes related to congenital immunity (CD3+CD8+, CD56+), and in patients in the cryotype, on the one hand subpopulation of lymphocytes related to adaptive immunity (CD3+), and on the other CD8+, which includes subpopulations of lymphocytes and congenital, and adaptive immunity. The conclusion. High specificity of these tests allows to identify patients with the prospect of pregnancy.CD56+CD3-CD8+CD8+CD3+repeated failures of implantationCD56 +CD3-CD8+CD8+CD3+diagnostic significancespecificitysensitivityповторные неудачи имплантацииgδ+Т-клеткидиагностическая значимостьспецифичностьчувствительностьgδ+Т cells[Coughlan C, Ledger W, Wang Q. et al. Recurrent implantation failure: definition and management. Reprod Biomed Online 2014; 28 (1): 14-38.][Pei-Yan Liang, Liang-Hui Diao, Chun-Yu Huang et al. The pro-inflammatory and anti-inflammatory cytokine profile in peripheral blood of women with recurrent implantation failure. Am J Reprod Immunol 2015; 73: 12-21.][Cухих Г.Т., Ванько Л.В. Иммунные факторы в этиологии и патогенезе осложнений беременности. Акуш. и гинекол. 2012; 1.][Степанова Е.О., Николаева М.А., Бабаян А.А. и др. Роль регуляторных клеток в формировании иммунной толерантности при беременности. Акуш. и гинекол. 2013; 2: 24-8.][Aluvihare V.R, Kallikourdis M, Betz A.G. Regulatory T cellsmediate maternal tolerance to the fetus. Nat Immunol 2004; 5: 266-71.][Arruvito L, Sanz M, Banham A., Fainboim L. Expansion of CD4+CD25+ and FOXP3+ regulatory T-cells during the follicular phase of the menstrual cycle: implications for human reproduction. J Immunol 2007; 178: 2572-8.][Nikahava H, Kato T, Tawara I et al. IFN-γ controls the generation/activation of CD4+CD25+ regulatory T-cell in antitumor immune response. J Immunol 2005; 175: 4433-40.][Fainboim L, Arruvito L, Sanz M, Alison H. Expansion of CD4+CD25+ and FOXP3+ Regulatory T Cells during the Follicular Phase of the Menstrual Cycle: Implications for Human Reproduction. J Immunol 2017; 178: 2572-8.][Vrekoussis T, Kalantaridou S.N., Mastorakos G. The role of stress in female reproduction and pregnancy:an update. Acad Sci 2010; 1205: 69-75.]