


No 7 (2023)
Experimental Articles. Soil Fertility
Replenishment of Humus due to Crop-Root Residues in Flax and Crop Rotations
Abstract
In the Central region of the Non-Chernozem zone of Russia on sod-podzolic light loamy soil in a long stationary experiment in flax crop rotations, the process of replenishing humus due to crop-root residues was studied. Alternation of crops in the crop rotation in 2004–2011: pure steam – winter rye with sowing of perennial grasses (red clover and timofeevka) – perennial grasses of the 1st year of use – perennial grasses of the 2nd year of use – flax – potato – barley – oats. In 2012–2018, potatoes were excluded from the crop rotation. The contribution of cultivated crops to the accumulation of soil organic matter varied. The largest amount of crop-root residues (6.8 t/ha) remained in the soil after harvesting perennial grasses, which provided a positive humus balance of +1.35 t/ha. In the crop rotation with potatoes without the use of fertilizers, the humus deficit was –0.20 t/ha, with the exclusion of potatoes from the crop rotation, the deficit decreased to –0.05 t/ha per year. The use of fertilizers increased the productivity of crop rotations by an average of 75%, the amount of crop-root residues increased by 10.6 t/ha or 58%, which allowed to obtain a positive balance of humus in the soil. The use of fertilizers at a dose of (NPK)200 (manure 5 t/ha + (NPK)135 and manure 5.7 t/ha + (NPK)120) in an 8-pole crop rotation with pure steam and potatoes when saturated by 25% with perennial grasses provided a humus balance of +0.17 t/ha per year, in 7-full crop rotation with pure steam and without potatoes at saturation by 28.5% with perennial grasses – +0.43 t/ha per year.



Agrochemical Conditions of Loamy Haplic Luvisol at Sour Cherry Orchard after 6-Year Treatments by Nitrogen and Potassium Fertilizers
Abstract
In a field experiment in a cherry orchard, the effect of annual application of urea and potassium sulfate on the most important agrochemical properties of the soil in the root zone was evaluated. The soil of the garden is medium-loamy agro-gray with initially favorable parameters: pHKCl 5.8, humus content – 3.58–4.57%. Fertilizers have been applied since 2017 1 time a year in early spring according to the scheme: 1 – control (without fertilizers), 2 – N30K40, 3 – N60K80, 4 – N90K120, 5 – N120K160. Soil samples were taken from soil layers 0–20, 20–40 and 40–60 cm in 2017 (the first year of experience) and in 2022 (after 6 years of fertilization). The annual application of fertilizers for 6 years led to a significant increase (by 1.4–2.2 times) in the availability of exchangeable potassium in the soil layer of 0–20 cm with a constant humus content in the entire root zone. At the same time, an increase in acidity and loss of calcium occurred in the upper layers of the soil under the influence of fertilizers in doses of N60K80 or more. For the period 2018–2022, the highest total yield of Turgenevka cherry trees was noted when applying fertilizers N60K80 and N120K160 (35.5 and 36.5 t/ha, respectively), but this increase was insignificant compared to the control (29.3 t/ha). Thus, an agro-gray medium loamy soil with favorable agrochemical characteristics can ensure the growth and fruiting of cherry trees without the use of fertilizers for 8 years after planting and at the same time the level of soil fertility remains stable.



Experimental Articles. Fertilizers
Transformation of the Botanical Composition of the Herbage with the Use of Mineral Fertilizers and Sowing Seeds on the Developed Peat Bog
Abstract
The results of a 5-year study of changes in the botanical composition of seeded and natural herbage, soil properties depending on the applied agricultural techniques, which included direct sowing of grasses into the turf of the developed peat bog and the use of mineral fertilizers, are presented. The introduction of mineral fertilizers contributed to the enrichment of the soil with available nutrients, which made it possible to increase the productivity of herbs in combination with sowing by 2.0–4.7 times, to improve their quality by optimizing the botanical composition of the herbage. It was found that the use of mineral fertilizers in doses of N60P60K90 and N60P90K120 in variants with natural and cultural phytocenoses provided an increase in the proportion of valuable legumes and cereals, reducing the proportion of various grasses from 53 to 25–34%.



Effect of Long-Term Use of Fertilizers on Soil-Dwelling Micromycetes of Meadow Chernozem Soil in Wheat Crops
Abstract
The effect of long-term systematic application of fertilizers on the mycobiota of meadow chernozem soil in a stationary field experiment (Amur region) was studied for the first time. Information about cultivated soil microscopic fungi is given, an annotated list including 26 species is compiled. It was revealed that the application of nitrogen fertilizers (N30) for wheat against the background of long-term use of fertilizers caused an increase in the total number of soil fungi relative to the control variant by an average of 30–68%, whereas with their aftereffect, a less significant increase in the number of mushroom germs was observed (23–29%). The intervals of the optimal content of macronutrients for the active increase of the pool of microscopic fungi in meadow chernozem soil were determined: phosphorus – 50–65, potassium – 195–210, nitrogen – ≥10 mg/kg with optimal soil moisture of 22–24%. The aftereffect of long-term application of fertilizers, especially organo-mineral fertilizers, as well as the application of mineral fertilizers (N30) directly in the year of the study had a positive effect on species diversity, frequency of occurrence and dynamics of the number of fungal germs in the soil. Negative rearrangements of micromycete complexes as a result of prolonged use of mineral fertilizers in the agrocenoses of meadow chernozem soils were not detected, the introduction of nitrogen-phosphorus fertilizers contributed to a significant decrease in the pool of phytopathogenic fungi.



Experimental Articles. Pesticides
Compositions of Fungicides with Growth Regulators which Reduce the Retarding Effect of Crop Protectants
Abstract
One of the reasons for the decrease in the yield of crops is the retardant effect of fungicides. In addition, many fungicides have a toxigenic effect on the mycelium of the fungus. The combination of a fungicide and a plant growth stimulant in a complex preparation can help to reduce the level of the negative effect of the pesticide. It is proposed to introduce individual growth regulators and their combination into the composition of the seed protectant in doses of 50, 100, 200 g/l. The length and the weight of the root and shoot of seedlings under the influence of the seed protectant were less than in the control, and the weight of the endosperm of grain was greater. Under the influence of the seed protectant and gibberellic acid, the length of the shoots corresponded to the control, but their weight was decreased. Indolylbutyric acid, added to the seed protectant, contributed to the effective consumption of spare substances of the endosperm of grain and root growth. Under the influence of the protectant and diphenylurea, the length and the weight of the shoot increased significantly in a balanced manner. The composition of the protectant and the named growth regulators in minimal doses affected the growth of the roots and shoots in the best way of all variants. Preference should be given to low doses of phytohormones and their combination.



Experimental Articles. Agroecology
Assessment of Greenhouse Gas Emissions and Carbon Reserves with Zero Processing of Chernozem in the Conditions of the Forest-Steppe Zone of the Middle Volga Region
Abstract
The transition from conventional soil treatment technology to zero treatment technology contributes to an increase in carbon sequestration (Sorg) in the form of carbon dioxide (CO2) from the atmosphere into the soil and, as a result, a reduction in the adverse effects of the greenhouse effect on the ecological state of the environment. The effectiveness of the application of zero tillage is to a greater or lesser extent due to specific agro-climatic conditions, crop rotation systems, fertilizers and plant protection, soil quality and stability. The influence of zero tillage on the dynamics of sorghum reserves and greenhouse gas emissions (CO2, N2O, CH4) in the agro-climatic conditions of crop production (LLC “Orlovka AIC”, Samara region) was investigated. The study was conducted on agrochernozem heavy loam in September–November 2021 in conditions of an abnormally arid growing season and high summer temperatures. On plots with zero tillage, plant residues were received: 268–1720 kg С/ha, 3–66 kg N, 0.2–7.7 kg P and 12–44 kg K/ha. Based on the results obtained, recommendations are proposed for further improving the efficiency of zero tillage technology due, firstly, to reducing its adverse effect on the density of soil composition, and, secondly, taking into account the influence of underlying parent rocks and relief on water erosion of soil and redistribution of granulometric fractions of soil in the agricultural landscape, which will allow using this technology according to principles of adaptive landscape farming.



Endophytic Strains of Bacillus thuringiensis for the Development of Means to Control the Number of the Colorado Potato Beetle in Potato Crops
Abstract
Currently, the search for B. thuringiensis strains to be used as biological control agents is based on their toxicity to insects in laboratory tests. At the same time, a number of strains of these bacteria are able to exist in symbiotic relationships with host plants, including being endophytes. The ability of B. thuringiensis strains to penetrate into the internal tissues of plants was evaluated by counting colony-forming units (CFU) of microorganisms 7 days after inoculation of sterile potato plants in test tubes; insecticidal activity was tested on larvae of the 3rd age obtained from overwintered adults of the Colorado beetle collected from fields in the Chishminsky and Iglinsky districts of Bashkortostan. It was shown that the strain of B. thuringiensis B-5351, which inhabits the surface (50.01 ± 8.10 CFU × 105/g) and internal tissues (38.92 ± 9.62 CFU × 105/g) of plant shoots, but has less insecticidal activity than the strain B. thuringiensis B-5689, which exhibits high insecticidal activity and colonizes mainly plant roots (25.37 ± 3.82 CFU × 105/g), reduced colonization of potato crops by the Colorado potato beetle, and also increased the yield of tubers in a 2-year experiment (2020–2021). Under the influence of B. thuringiensis B-5351, a decrease in the number of larvae of early age was observed, in contrast to B. thuringiensis B-5689, which caused longer metamorphosis processes. Apparently, the effect of B. thuringiensis B-5351 was the death of insects. It is important that processing B. thuringiensis B-5351 contributed to a significant decrease in the number of late-instar larvae on crops treated with this strain, as well as the degree of defoliation of plants by the pest, which was not observed with the action of B. thuringiensis B-5689. On plots treated with B. thuringiensis B-5351, the yield of commercial tubers and the total yield increased. A method is proposed for studying the endophytic potential of strains in relation to the terrestrial part of plants to search for biocontrol agents as a basis for creating algorithms for constructing microbiomes in agrocenoses.



Edge Treatment of the Field and Field-Protective Forest Plants from Weeds and Pests
Abstract
The design of the device for a hinged rod sprayer of plants for edge processing of the field by the main method of spraying of litter and pests is proposed. The average number of droplets deposited on the surface per 1 cm2 at a distance of up to 15 m meets the agrotechnical requirements for the use of herbicides, as well as insecticides and fungicides. According to the height of the cards at a distance of 10 m from the nozzle, the number of drops per 1 cm2 decreased, and especially with an increase in the height of their location from 69.7 to 17.5 and 31.7 pcs/cm2. At a distance of 15 m from the nozzle, the droplets were small and practically did not settle on the cards. When the vehicle is moving 7.2 km/h (2.0 m/s), the total mass flow of the working fluid of 2 slot sprayers LU-04.AD-04 (color code – red) and 2 slot sprayers LU-02.AD-02, (color code – yellow) at a pressure of 4 The bar was 45.5 g/m linear. It took 45.5 liters of working fluid to work 1 km of the forest belt. For example, a capacity of 600 liters is sufficient for edge processing along the perimeter of a field with a length of 13.2 km. The developed technical means will ensure the effective treatment of protective forest plantations from weeds and pests.



Experimental Articles. Ecotoxicology
Monitoring of 37C, 90Sr and 40Kin Sod-Podzolic Soils and Plants of Reference Sites of the Ivanovo Region
Abstract
The results of radiation monitoring of sod-podzolic soils of reference agricultural plots of the Ivanovo region, conducted in 2014 and 2021, are presented. Background values of the specific activities of the studied radionuclides in the soils of reference sites have been established. According to the pollution density of the arable horizon of soils 137Cs and 90Sr, the level of the ecological situation was determined, which made it possible to attribute the soils of all reference areas of the region to uncontaminated territories suitable without restrictions for agricultural use. The power of the exposure dose of gamma radiation of soils and the contribution of 40K and 137Cs to the formation of the gamma background of soils were determined. Correlation analysis determined the influence of individual physical and chemical properties of soils on the behavior and distribution of radionuclides in the soil and the relationship between the content of the radionuclides themselves. Based on the values of the specific activity of 137Cs and 90Sr in plant products grown in 2014 and 2021, rows of cultivated plants with a decreasing ability to accumulate these radionuclides from the soil were constructed. All grown plant products of all types of crops fully met the veterinary standards required to limit the content of 137Cs and 90Sr in plant feeds (green mass, straw and feed grain). The coefficients of accumulation and transition of 137Cs and 90Sr from soil to plants are calculated. The coefficients of linear correlation between the values of the specific activity of 137Cs and 90Sr in soils and plants revealed the strength and nature of these dependencies.



Reviews
Agrogeochemical Technologies for Managing CO2 Flows in Agroecosystems. Message 2. Restoration of the Microbial Link of the Agrogeochemical Cycle
Abstract
The application of agrogeochemical technologies aimed at restoring agrogeochemical cycles in agricultural ecosystems, primarily in the microbial link regulating CO2 flows, is considered. Examples of recultivation of disturbed and polluted soils, waterlogged and/or over-dried soils are given and their impact on the amount of CO2 flux is estimated. A complex of agrogeochemical technologies aimed at assessing and stabilizing the microbial link of the biogeochemical cycle in agroecosystems is presented. Examples of the use of these technologies for the regulation of CO2 emissions in agroecosystems are given. Using one of these technologies, an almost 5-fold decrease in the rate of CO2 flows during the reclamation of disturbed pasture ecosystems of the tundra is shown. It is necessary to further develop and use agrogeochemical technologies aimed at restoring biogeochemical cycles in agroecosystems, primarily in the microbial link regulating CO2 flows.


