The first-ever in-Russia discoveries of the entomopathogenic fungus Akanthomyces uredinophilus from the Republic of Adygea

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

The first finding of cryptic entomopathogenic fungus Akanthomyces uredinophilus (Ascomycota, Hypocreales, Cordycipitaceae) in Russia is reported. Specimens were found in 2019 and 2022 in the North Caucasus at two 60 km distant points in the Republic of Adygea (1st – southern outskirts of Maykop, wooded damp ravine, N44°34ʹ24ʺ E40°08ʹ54ʺ, 240 m a. s. l.; 2nd – vicinity of Nickel settlement, Syuk River gorge, N44°10ʹ46ʺ E40°09ʹ29ʺ, 570 m a. s. l.) on dead insects: alien North American species of сitrus flatid planthopper Metcalfa pruinosa (Hemiptera, Auchenorrhyncha, Flatidae), whitefly Aleyrodes lonicerae (Hemiptera, Aleyrodidae), psyllid (Hemiptera, Psylloidea), and cranefly (Diptera, Tipuloidea). We have demonstrated high morphological and genetic (five gene loci EF1A, RPB1, RPB2, LSU and SSU) similarity of Adygean isolates with the type strain KACC44082 (Republic of Korea) from uredinia of the rust fungus Pucciniastrum agrimoniae and other isolates of Akanthomyces uredinophilus previously found in China and Argentina on dead insects. Under laboratory conditions all four Adygean isolates showed high virulence against larvae of population of green peach aphid Myzus persicae (Hemiptera, Aphididae) feeding on Vicia faba under experimental conditions including 25 ± 1°C temperature and spraying suspension with a spores (conidia) concentration 107 per 1 ml aphid mortality ranged from 30% [isolate MPi-Ad(Mp)19→Ak6] to 89% (isolate HPs – Ad22→B10) after three days. After seven days the mortality reached 100% in all variants, while it was only 15% in the control sample. This indicates the expediency of further study of these isolates as potential producers of commercial mycoinsecticides against harmful phytophagous insects.

Толық мәтін

Рұқсат жабық

Авторлар туралы

M. Cherepanova

All-Russian Institute of Plant Protection

Хат алмасуға жауапты Автор.
Email: cherepma@mail.ru
Ресей, Saint Petersburg, 196608

G. Mitina

All-Russian Institute of Plant Protection

Email: galmit@rambler.ru
Ресей, Saint Petersburg, 196608

A. Choglokova

All-Russian Institute of Plant Protection

Email: 4oglik@inbox.ru
Ресей, Saint Petersburg, 196608

B. Borisov

AgroBioTechnology LLC

Email: borborisov@mail.ru
Ресей, Moscow, 125212

Әдебиет тізімі

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2. Fig. 1. Map-scheme of the Republic of Adygea with designation of the surveyed territories (black triangles) and locations of Akanthomyces uredinophilus fungus findings (white squares).

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3. Fig. 2. Findings of Akanthomyces uredinophilus in the Republic of Adygea: (a) - site of fungal epizootic of white cicada in the vicinity of Maikop; (b-d) - cicadas affected by the fungus; (e) - cicada with smoky-pink plaque of Fusarium sp. fungus. (for comparison); (f) - honey bee affected by the fungus; (g) - location of the fungus on a long-legged mosquito in the vicinity of Nickel settlement; (h) - a long-legged mosquito killed by mycosis; (i) - conidiogenic structures of MPi-Ad(Mp)19→Ak6 isolate; (j) - growth of the isolate on agarised semi-synthetic nutrient medium after 15 days.

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4. Fig. 3. Characteristic appearance of Akanthomyces uredinophilus colonies on the example of isolate MPi-Ad(Mp)19 → Ak6: (a) - top side; (b) - reverso; (c) - conidia. The scale is 5 µm.

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5. Fig. 4. Phylogenetic tree for the combined nucleotide sequences of EF1A, RPB1, RPB2, LSU, and SSU genes constructed by the maximum likelihood (ML) method. End branches are labelled with genus, species, and strain designations; ‘Type’ indicates the type strains of these species. Numbers on the inner branches indicate the percentage of initial bootstrap support. The dimensional scale corresponds to 1% sequence divergence along the length of the phylogram branches. The concatenation of sequences of the entomopathogenic fungus Beauveria bassiana (Hypocreales, Cordycipitaceae) was used as an external group.

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