Effect of the Bacteriocin Subtylosin P19 on Bacillus Anthracis Spores

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Abstract—

This is the first report on electron microscopic investigation of the effect of bacteriocin on Bacillus anthracis dormant spores. The new bacteriocin, subtilosin P19, isolated from a natural Bacillus subtilis strain, effectively killed dormant B. anthracis spores at concentrations of 0.5‒35 µg/mL after 60-min exposure. Our data, combined with analysis of the known mechanisms of the action of bacteriocins, suggest that the mechanism of spore inactivation was apparently based on multiple disruptions of the integrity of the inner and outer membranes surrounding the cortex of the resting spore. It is also likely that the molecular configuration of subtilisin P19 allows it to penetrate through the outer integuments and to damage the vital membrane structures of the anthrax spores. Subtilosin P19 can serve as a promising basis for new antimicrobial preparations of natural origin.

Sobre autores

V. Pokhilenko

State Research Center for Applied Microbiology and Biotechnology

Autor responsável pela correspondência
Email: pokhilenko@obolensk.org
Russia, 142279, Obolensk

V. Gerasimov

State Research Center for Applied Microbiology and Biotechnology

Email: pokhilenko@obolensk.org
Russia, 142279, Obolensk

S. Zhigletsova

State Research Center for Applied Microbiology and Biotechnology

Email: pokhilenko@obolensk.org
Russia, 142279, Obolensk

T. Kalmantaev

State Research Center for Applied Microbiology and Biotechnology

Email: pokhilenko@obolensk.org
Russia, 142279, Obolensk

I. Chukina

State Research Center for Applied Microbiology and Biotechnology

Email: pokhilenko@obolensk.org
Russia, 142279, Obolensk

R. Mironova

State Research Center for Applied Microbiology and Biotechnology

Email: pokhilenko@obolensk.org
Russia, 142279, Obolensk

А. Gajtrafimova

State Research Center for Applied Microbiology and Biotechnology

Email: pokhilenko@obolensk.org
Russia, 142279, Obolensk

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Declaração de direitos autorais © В.Д. Похиленко, В.Н. Герасимов, С.К. Жиглецова, Т.А. Калмантаев, И.А. Чукина, Р.И. Миронова, А.Р. Гайтрафимова, 2023