Induction of alcohol dehydrogenase in Yarrowia lipolytica yeast under stress conditions

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It has been shown for the first time that under stress conditions, the yeast Yarrowia lipolytica exhibits activity of alcohol dehydrogenase along with antioxidant enzymes. During growth on glucose, during the transition of yeast from the exponential to the stationary growth phase, caused by glucose depletion, the ability of cells to oxidize ethanol was detected. Under conditions of oxidative and thermal stress, the induction of alcohol dehydrogenase is associated with an increase in the activity of antioxidant enzymes, in particular, catalase, superoxide dismutase, glucose-6-phosphate dehydrogenase and glutathione reductase. A decrease in the intracellular level of ATP and cAMP in Y. lipolytica under various stress conditions was shown, which correlates with an increase in the activity of antioxidant systems and NAD+-dependent alcohol dehydrogenase.

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Sobre autores

E. Biryukova

Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”

Autor responsável pela correspondência
Email: biryukovae05@rambler.ru

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences

Rússia, Pushchino, Moscow Region, 142290

A. Arinbasarova

Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”

Email: biryukovae@rambler.ru

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences

Rússia, Pushchino, Moscow Region, 142290

A. Medentsev

Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”

Email: biryukovae05@rambler.ru

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences

Rússia, Pushchino, Moscow Region, 142290

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2. Figure. Ethanol oxidation by Y. lipolytica cells grown on glucose and ethanol: 1 ‒ from the exponential growth phase (exp.); 2 ‒ from the stationary growth phase (station.); 3 ‒ treated with 0.5 mM H2O2; 4 ‒ incubated at 37°C for 60 min; 5 ‒ grown on ethanol. The numbers on the curves indicate the oxygen consumption activity, nmol O2 /min/mg dry cells.

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