Features of development of microscopic fungi in conditions of ultra-weak magnetic fields

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Abstract. The paper presents the results of a study of the effect of ultra-weak magnetic fields (MF) on the viability, growth characteristics, respiratory activity, and antagonistic properties of microscopic fungi. The experiments were conducted on strains isolated from the interior of the International Space Station. To create hypomagnetic conditions (HMC), the hypomagnetic chambers GMK-1 and GMK-2, shielding the Earth’s MF, were used in the experiments. The chamber walls are a two-section magnetic screen made of amorphous permalloy tape. In the experiments, the GMK chambers made it possible to reduce the geomagnetic field by 1000–2000 times. The maximum value of the MF after demagnetization did not exceed 45 nT. It was found that the hypomagnetic field (HMC) did not have a predominantly inhibitory and/or stimulating effect on the viability of spores and the growth of fungal colonies, as indicated by the absence of reliable changes in the quantitative level, percentage of spore germination and radial growth rate of the tested strains in the HMC compared to geomagnetic conditions. At the same time, the growth and respiration rate of micromycetes in some cases was significantly stimulated in the GMF during their development on the surface of samples of structural materials under conditions of limited availability of nutrients. It was also found that the GMF affects the antagonistic properties of some microscopic fungi. The Penicillium rugulosum 633.12 strain grown in the GMF completely lost its antagonistic activity towards bacteria, which was found to be high when cultivated under standard geomagnetic conditions. The results obtained are discussed in the context of the features of microbial colonization of the habitat of future lunar complexes.

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Sobre autores

V. Rodimin

Institute of Biomedical Problems of the Russian Academy of Sciences

Email: charin@imbp.ru
Rússia, Moscow, 123007

S. Kharin

Institute of Biomedical Problems of the Russian Academy of Sciences

Autor responsável pela correspondência
Email: charin@imbp.ru
Rússia, Moscow, 123007

S. Poddubko

Institute of Biomedical Problems of the Russian Academy of Sciences

Email: charin@imbp.ru
Rússia, Moscow, 123007

A. Kurakov

Lomonosov Moscow State University

Email: charin@imbp.ru

Faculty of Biology

Rússia, Moscow, 119234

S. Kulachkova

Lomonosov Moscow State University

Email: charin@imbp.ru

Faculty of Biology

Rússia, Moscow, 119234

M. Yarmeeva

Lomonosov Moscow State University

Email: charin@imbp.ru

Faculty of Biology

Rússia, Moscow, 119234

V. Lebedev

Lomonosov Moscow State University

Email: charin@imbp.ru

Skobeltsyn Research Institute of Nuclear Physics

Rússia, Moscow, 119991

A. Spassky

Lomonosov Moscow State University

Email: charin@imbp.ru

Skobeltsyn Research Institute of Nuclear Physics

Rússia, Moscow, 119991

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2. Fig. 1. The number of viable spores of test cultures of microscopic fungi when crops are kept in a standard geomagnetic field (control) and a GMF of no more than 45 nT (experiment) for 4, 6 and 9 days

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3. Fig. 2. Percentage of spore germination of test cultures of microscopic fungi when kept in a standard geomagnetic field (control) and a GMF of no more than 45 nT (experiment) for 4, 6 and 9 days

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4. Fig. 3. Radial growth rate of colonies of test cultures of microscopic fungi during their cultivation in a standard geomagnetic field (control) and a GMF of no more than 45 nT (experiment) for 4, 6 and 9 days

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5. Fig. 4. Score assessment of the growth of test cultures of microscopic fungi on samples of structural materials after 14 and 28 days of exposure in a standard geomagnetic field (control) and a GMF of no more than 45 nT (experiment).

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6. Fig. 5. Production of carbon dioxide by test cultures of microscopic fungi developing on samples of structural materials in a standard geomagnetic field (control) and a GMF of no more than 45 nT (experiment).

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