HPLC-MS/MS-determination of antihistamines cyproheptadine, diphenhydramine and tripelenamine in foods and feeds

封面

如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅或者付费存取

详细

The method of determination of antihistamine substances cyproheptadine, diphenhydramine and tripelenamine in food and feed by high-performance liquid chromatography-tandem mass spectrometry was proposed. When developing the method, its versatility in terms of reagents and equipment used in the analysis of matrices of different types – meat, offal (liver), eggs, milk, honey, butter, as well as mixed fodder – was considered as the main aspect. A combination of solvents was used for the isolation of analytes: acetonitrile, water and hexane (3 ml of each), the sequence of solvent addition depended on the matrix, the rest of the sample preparation steps were unified. The limits of detection and determination of antihistamines in the above objects were 0.012-0.3 and 0.04-1 μg/kg, respectively. The relative standard deviation of the analytical results does not exceed 17 %. The relative error of determination of substances in most cases does not exceed 15 %.

全文:

受限制的访问

作者简介

A. Melekhin

Federal Center for Animal Health

编辑信件的主要联系方式.
Email: nikatolm@mail.ru
俄罗斯联邦, Vladimir

V. Tolmacheva

Federal Center for Animal Health; Lomonosov Moscow State University, chemistry department

Email: nikatolm@mail.ru
俄罗斯联邦, Vladimir; Moscow

A. Bairov

Federal Center for Animal Health

Email: nikatolm@mail.ru
俄罗斯联邦, Vladimir

E. Usanova

Federal Center for Animal Health

Email: nikatolm@mail.ru
俄罗斯联邦, Vladimir

T. Penkov

Federal Center for Animal Health

Email: nikatolm@mail.ru
俄罗斯联邦, Vladimir

V. Tishchenko

Federal Center for Animal Health; Peoples' Friendship University of Russia

Email: nikatolm@mail.ru
俄罗斯联邦, Vladimir; Moscow

V. Apyari

Lomonosov Moscow State University, Chemistry department

Email: nikatolm@mail.ru
俄罗斯联邦, Moscow

参考

  1. Полосьянц О.Б. Обзор антигистаминных средств первого и второго поколений, рациональный подход к использованию в клинической практике // Лечащий врач. 2011. № 07. С. 66.
  2. Zheng Z., Li X., Liu H., Gao Y. Comparison of clonidine and cyproheptadine determination in animal-derived foods by sweeping-micellar electrokinetic chromatography and large volume sample stacking-capillary zone electrophoresis // Acta Chromatogr. 2022. V. 34. P. 461.
  3. Decheng S., Xia F., Zhiming X., Yang L., Shi W. Simultaneous determination of 10 first-generation histamine H1 receptor blockers in feeds by ultra-high-performance liquid chromatography triple quadrupole ion trap hybrid mass spectrometer // J. Sep. Sci. 2023. V. 46. Article 2200840.
  4. https://residue.foodsafetykorea.go.kr/vd/mrl (20.03.2025 г)
  5. Yang J., Wang Z., Zhou T., Song X., Liu Q., Zhang Y., He L. Determination of cyproheptadine in feeds using molecularly imprinted solid-phase extraction coupled with HPLC // J. Chromatogr. B. 2015. V. 990. P. 39.
  6. Feás X., Ye L., Hosseini S.V., Fente C.A., Cepeda A. Development and validation of LC–MS/MS method for the determination of cyproheptadine in several pharmaceutical syrup formulations // J. Pharm. Biomed. Anal. 2009. V. 50. P. 1044.
  7. Abdelrahman M.M., Abdelaleem E.A., Ali N.W., Emam R.A. Development and validation of stability indicating high-performance liquid chromatographic method for determination of cyproheptadine hydrochloride, its impurity and degradation product // J. Chromatogr. Sci. 2021. V. 59. № 2. P. 128.
  8. Sy N.V., Dzung L.H. Validation of LC-MS/MS method for determination of cyproheptadine in dietary supplements // VN J. Nutr. Food. 2022. V. 18(2E). P. 9.
  9. Dewi M.S., Riswanto F.D.O., Yuliani S.H. Analytical method validation and simultaneous determination of ketotifen fumarate and cyproheptadine hydrochloride in divided powder by RP-HPLC-UV method // Indones. J. Pharm. 2024. V. 35. № 3. P. 549.
  10. Omar M.A., Moustafa M.A.H., Almaghrabi M., Aljohani A.K.B., Attia T.Z. Validated spectrofluorimetric method for determination of cyproheptadine in pharmaceutical formulation and human plasma. Application to content uniformity test // Results Chem. 2024. V. 7. Article 101341.
  11. Kim J.Y., Choi J.Y., Yoon C.Y., Cho S., Kim W.S., Do J.A. LC–MS/MS monitoring of 22 illegal antihistamine compounds in health food products from the Korean market // J. Korean Soc. Appl. Biol. Chem. 2015. V. 58. № 1. P. 137.
  12. Стрелков С.В., Подушкин В.Ю., Котова Н.И. Валидация аналитической методики количественного определения дифенгидрамина гидрохлорида в препарате аптечного изготовления “Антигриппин для взрослых № 12” // Успехи современной науки 2017. Т. 1. № 8. С. 84.
  13. Мирзаева Х.А., Гамзаева У.Г. Ионный ассоциат молибден(VI)–галлеин–димедрол и его применение для экстракционно-фотометрического определения димедрола // Журн. аналит. химии. 2018. Т. 73. № 7. С. 508. (Mirzaeva Kh.A., Gamzaeva U.G. A molibdenum(VI)–gallein–diphenhydramine ion associate and its application to the extraction-photometric determination of diphenhydramine // J. Anal. Chem. 2018. V. 73. № 7. P. 661.)
  14. Османова М.М., Мирзаева Х.А. Определение димедрола в лекарственных препаратах методом твердофазной спектрометрии // Заводск. лаборатория. 2017. Т. 83. № 12. С. 21. (Osmanova M.M., Mirzaeva Kh.A. Determination of diphenhydramine hydrochloride in pharmaceuticals using solid-phase spectrophotometry // Ind. Lab. Diagn. Mater. 2017. V. 83. № 12. P. 21 (in Russ.))
  15. Темердашев А.З., Киселева Н.В., Матвиенко В.Г. Определение некоторых наркотических средств природного и синтетического происхождения с использованием методов хромато-масс-спектрометрии // Аналитика и контроль. 2014. Т. 18. № 3. С. 266.
  16. Слустовская Ю.В., Крысько М.В., Стрелова О.Ю., Куклин В.Н. Исследование волос с целью диагностики употребления психоактивных веществ // Вестник Российской военно-медицинской академии. 2019. Т. 21. № 1. С. 120.
  17. Kudo K., Saka K., Waters B., Hara K., Matsusue A., Kashiwagi M., Kubo S. Effects of putrefaction on the quantitative analyses of diphenhydramine in blood and tissues: model experiments by the routine matrix-matched calibration method and standard addition method with different internal standards // Forensic Toxicol. 2021. V. 39. P. 437.
  18. Feás X., Ye L., Regal P., Fente C.A., Hosseini S.V., Cepeda A. Application of dummy molecularly imprinted solid-phase extraction in the analysis of cyproheptadine in bovine urine // J. Sep. Sci. 2009. V. 32. P. 1740.
  19. Fente C.A., Regal P., Vázquez B.I., Feás X., Franco C.M., Cepeda A. Development and validation of an LC-MS/MS confirmatory method for residue analysis of cyproheptadine in urine of food-producing animals // J. Agric. Food Chem. 2009. V. 57. P. 2595.
  20. Wang X., Wang K., Xu Z., Yang S., Zhao Y. Development and validation of a multi-residue analytical method for veterinarian and human pharmaceuticals in livestock urine and blood using UHPLC-QTOF // J. Chromatogr. B. 2021. V. 1167. Article 122564.
  21. Тимакова С.И., Симакина Я.И., Кравец К.Ю., Гречников А.А. Экспресс-анализ проб мочи методом масс-спектрометрии с ионизацией, индуцированной лазерной плазмой при атмосферном давлении // Аналитика и контроль. 2023. Т. 27. № 3. С. 141.
  22. Thompson H.C., Jr., Holder C.L., Althaus J.R. Trace determination of the antihistamines tripelennamine hydrochloride, thenyldiamine hydrochloride, and chlorothen citrate in admixture in animal feed, human urine, and wastewater by high-pressure liquid chromatography and use of a fluorescence detector // Talanta. 1983. V. 30. № 4. P. 251.
  23. Tanoue R., Nozaki K., Nomiyama K., Kunisue T., Tanabe S. Rapid analysis of 65 pharmaceuticals and 7 personal care products in plasma and whole-body tissue samples of fish using acidic extraction, zirconia-coated silica cleanup, and liquid chromatography-tandem mass spectrometry // J. Chromatogr. A. 2020. V. 1631. Article 461586.
  24. Katselou M., Athanaselis S., Nikolaou P., Qammaz S., Dona A., Spiliopoulou C., Papoutsis I. A fully validated method for the simultaneous determination of 11 antihistamines in breast milk by gas chromatography-mass spectrometry // Biomed. Chromatogr. 2018. V. 32. № 8. Article e4260.
  25. Park J., Zhang D., Kim D.-S., Kim S.-K., Cho K.-S., Jeong D., Shim J.-H., Kim J.-S., El-Aty A.M.A., Shin H.-C. Single-step multiresidue determination of ten multiclass veterinary drugs in pork, milk, and eggs using liquid chromatography with tandem mass spectrometry // J. Sep. Sci. 2015. V. 38. P. 2772.
  26. Jung Y.S., Kim D.-B., Nam T.G., Seo D., Yoo M. Identification and quantification of multi-class veterinary drugs and their metabolites in beef using LC–MS/MS // Food Chem. 2022. V. 382. Article 132313.
  27. Дмитриенко С.Г., Апяри В.В., Толмачева В.В., Горбунова М.В., Фурлетов А.А., Золотов Ю.А. Методы выделения органических соединений из твердых образцов. 1. Жидкостная экстракция. Обзор обзоров // Журн. аналит. химии. 2024. Т. 79. № 8. С. 811. (Dmitrienko S.G., Apyari V.V., Tolmacheva V.V., Gorbunova M.V., Furletov A. A., Zolotov Yu.A. Methods for the extraction of organic compounds from solid samples: 1. Solvent extraction. Review of reviews // J. Anal. Chem. 2024. V. 79. № 8. P. 999.)
  28. Дмитриенко С.Г., Апяри В.В., Толмачева В.В., Горбунова М.В., Фурлетов А.А., Цизин Г.И., Золотов Ю.А. Методы выделения органических соединений из твердых образцов. 2. Экстракция в суб- и сверхкритических условиях. Матричная твердофазная дисперсия. Метод QUECHERS. Обзор обзоров // Журн. аналит. химии. 2024. Т. 79. № 9. С. 935. (Dmitrienko S.G., Apyari V.V., Tolmacheva V.V., Gorbunova M.V., Furletov A.A., Tsizin G.I., Zolotov Yu.A. Methods for extraction of organic compounds from solid samples: 2. Sub- and supercritical Extraction. Matrix solid-phase dispersion. QuEChERS method. Review of reviews // J. Anal. Chem. 2024. V. 79. № 9. P. 1167.)
  29. Bhojya Naik H.S., Venugopala Reddy K.R. Synthetic, spectral and thermal studies of Cobalt(II) complexes with diphenhydramine // Rev. Inorg. Chem. 2009. V. 29. № 3. P. 157.
  30. Ware R., Jadhav R., Joshi H., Thakur S. Complexation of zinc metal with medicinal drugs in mixed solvent media // Int. J. Sci. Res. Sci. Technol. 2024. V. 11. № 21. P. 58.

补充文件

附件文件
动作
1. JATS XML
下载
2. Fig. 1. Sample preparation scheme for different types of samples for the determination of antihistamines.

下载 (439KB)
索引源数据
3. Fig. 2. Structural formulas of cyproheptadine (a), diphenhydramine (b), and tripelennamine (c).

下载 (38KB)
索引源数据
4. Fig. 3. Effect of sample preparation method on chromatographic peak areas during extraction of antihistamines from meat.

下载 (82KB)
索引源数据
5. Fig. 4. Matrix effects (ME) in the determination of antihistamines in various matrices.

下载 (69KB)
索引源数据
6. Fig. 5. Mass chromatograms for selected ions of cyproheptadine (a), diphenhydramine (b), and tripelennamine (c) in a meat extract (10 µg/kg).

下载 (173KB)
索引源数据

版权所有 © Russian Academy of Sciences, 2025