Features of erythropoiesis of hibernating ground squirrels Urocitellus Undulatus

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Resumo

The morphofunctional features of the bone marrow of the femur and humerus in long-tailed ground squirrels were studied in summer, fall, during torpor and during periods of short-term awakenings in winter (winter euthermia). Histological analysis showed an increase in the number and size of adipocytes in the bone marrow in animals in the torpor state, with partial replacement of myeloid tissue by adipose tissue. Despite the greater volume of bone marrow in the femur, significantly fewer nuclear cells were found in the bone marrow than in the humerus, but significantly more erythroid islets were found, especially during hibernation. In torpid ground squirrels there is a replacement of disc-shaped erythrocytes in the blood by atypical forms of erythrocytes (oval, macrocytes, and michenoid), the number of which decreases significantly during the winter euthermal period. The level of reticulocyte content increases in the hibernation period twofold compared to summer. The results obtained are discussed in the context of maintaining high blood oxygen levels during torpor and adaptation of erythropoiesis to conditions of prolonged hypothermia.

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Sobre autores

K. Lizorkina

Institute of Cell Biophysics RAS - separate subdivision of Federal State Budgetary Institution of Science Federal Research Centre ‘Pushchino Scientific Centre for Biological Research RAS’

Autor responsável pela correspondência
Email: lizorkina_kseniia@mail.ru
Rússia, Pushchino, Moscow Region

G. Aksenova

Institute of Cell Biophysics RAS - separate subdivision of Federal State Budgetary Institution of Science Federal Research Centre ‘Pushchino Scientific Centre for Biological Research RAS’

Email: lizorkina_kseniia@mail.ru
Rússia, Pushchino, Moscow Region

V. Afanasyev

Institute of Cell Biophysics RAS - separate subdivision of Federal State Budgetary Institution of Science Federal Research Centre ‘Pushchino Scientific Centre for Biological Research RAS’

Email: lizorkina_kseniia@mail.ru
Rússia, Pushchino, Moscow Region

P. Teplova

Institute of Cell Biophysics RAS - separate subdivision of Federal State Budgetary Institution of Science Federal Research Centre ‘Pushchino Scientific Centre for Biological Research RAS’

Email: lizorkina_kseniia@mail.ru
Rússia, Pushchino, Moscow Region

N. Zakharova

Institute of Cell Biophysics RAS - separate subdivision of Federal State Budgetary Institution of Science Federal Research Centre ‘Pushchino Scientific Centre for Biological Research RAS’

Email: lizorkina_kseniia@mail.ru
Rússia, Pushchino, Moscow Region

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2. Fig. 1. Microphotographs of bone marrow preparations of long-tailed ground squirrels Urocitellus undulatus in active summer, autumn and torpid states.

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3. Fig. 2. The number of erythroid islets in the CM of both bones in the active summer, autumn, torpid and winter euthermic (WE) states of ground squirrels.

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4. Fig. 3. Percentage of nucleated cells in the S phase of the cell cycle in the bone marrow of (a) the humerus according to [1] and (b) the femur of ground squirrels in the active summer, torpid and winter euthermic states (n = 11 summer, n = 12 torpor, n = 11 EE).

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5. Fig. 4. Changes in the number of nucleated cells *10⁸/g bone marrow tissue of long-tailed ground squirrels in the humerus (light columns) and femur (dark columns) in the active summer, torpid and winter euthermic (WE) states (n = 7 for each state)

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6. Fig. 5. Micrographs of blood smear preparations from long-tailed ground squirrels Urocitellus undulatus. Diff-Quick staining.

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7. Fig. 6. Seasonal changes in the number of reticulocytes relative to the total content of erythrocytes in the peripheral blood of ground squirrels Urocitellus undulatus (n=6 for each studied condition)

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