Comparative characterization of Kupffer cells in the livers of SHR and Wistar rats

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Аннотация

In the present study, the structural features of resident liver macrophages were analyzed in the context of sustained arterial hypertension, compared to a normotensive control group. To identify resident macrophages in liver samples from nine-month-old male SHR and Wistar rats (n = 14), immunohistochemical staining against the Iba-1 protein was employed. Morphometric parameters and the spatial distribution patterns of Kupffer cells were assessed using the mathematical processing and image analysis software ImageJ and GIMP. It was shown that Kupffer cells in liver samples from SH rats predominantly exhibit a poorly branched or ellipsoidal shape and do not display a direct correlation with their location within the hepatic acinus, in contrast to macrophages from the Wistar group. Statistically significant differences were observed in the distribution patterns of Kupffer cells: in the SHR group, cells were distributed more uniformly within the hepatic acinus compared to those in the Wistar group, where the highest density of distribution was observed in the intermediary zone of the acinus. Identified structural and functional characteristics of resident liver macrophages in SH rats may be attributed to the functional disturbances in the liver associated with sustained arterial hypertension.

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Авторлар туралы

I. Nikitina

Institute of Experimental Medicine

Хат алмасуға жауапты Автор.
Email: inga06819@gmail.com
Ресей, Saint Petersburg

V. Razenkova

Institute of Experimental Medicine

Email: inga06819@gmail.com
Ресей, Saint Petersburg

D. Korzhevskii

Institute of Experimental Medicine

Email: inga06819@gmail.com
Ресей, Saint Petersburg

Әдебиет тізімі

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2. Fig. 1. Iba-1-immunopositive structures in liver preparations: (a, c) — in the liver of an SHR rat, microscope magnification x10 (a) and x100 (c); (b, d) — in the liver of a Wistar rat, microscope magnification x10 (b) and x100 (d). Asterisk — central vein; filled arrow — Kupffer cells with pronounced cytoplasmic processes; transparent arrow — ellipsoid-shaped Kupffer cells. Objectives Plan 10x/0.22 (a; b); Plan 100x/1.25 (c; d).

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3. Fig. 2. Structure of liver triads in a liver preparation: (a, c) — in the liver of an SHR rat; (b; d) — in the liver of a Wistar rat. Histological staining of preparations with aniline blue (a; b), hematoxylin and eosin (c; d). Objective Plan 40x/0.65.

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4. Fig. 3. Result of statistical comparison of Iba-1-immunopositive structures of two groups (SHR n = 7; Wistar n = 7) of structures.

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5. Fig. 4. Evaluation of the uniformity of distribution of immunopositive structures in images

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