Conjugation Vector Delivery System for Molecular Cloning into Cells of Bacteria of The Genus  Bacillus

A. S. Gurinovich; Гуринович А. С.; I. A. Fedyushko; Федюшко И. А.; М. А. Titok; Титок М. А.

doi:10.31857/S0555109923060065

Conjugation Vector Delivery System for Molecular Cloning into Cells of Bacteria of The Genus Bacillus

Авторлар: Gurinovich A.S.¹, Fedyushko I.A.¹, Titok М.А.¹
Мекемелер:
1. Department of Microbiology, Biological Faculty, Belarusian State University
Шығарылым: Том 59, № 6 (2023)
Беттер: 581-588
Бөлім: Articles
URL: https://gynecology.orscience.ru/0555-1099/article/view/674588
DOI: https://doi.org/10.31857/S0555109923060065
EDN: https://elibrary.ru/CVNJOP
ID: 674588

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат
Рұқсат жабық

Рұқсат берілді
Рұқсат жабық

Тек жазылушылар үшін

Аннотация
Авторлар туралы
Әдебиет тізімі
Қосымша файлдар
Статистика

Аннотация

A delivery system for vectors for molecular cloning into bacterial cells of the genus Bacillus has been developed. A specific feature of the developed system is the use of the pBS72 plasmid, which provides the conjugative transfer of the conditionally lethal vector pKS1mob obtained into the cells of the studied bacteria. The ability of vector pKS1mob to replicate in Escherichia coli and B. subtilis cells at a low temperature (30°C), the presence of two polylinkers around the kanamycin resistance gene makes it possible to clone target gene fragments into its composition using traditional genetic engineering approaches. Inactivation of the rok gene in the pBS72 plasmid made it possible to transform the strain containing it with the constructed vector pKS1mob with high efficiency. Crossing the donor strain B. subtilis 168, containing conjugative pBS72 and pKS1mob mobilizable plasmids, with a recipient strain of the genus Bacillus made it possible to introduce the pKS1mob plasmid into it. The possibility of using the created system for inactivation of gene codY of bacteria Bacillus licheniformis was shown.

Негізгі сөздер

B. subtilis, pBS72 plasmid, Rok protein, vector pKS1mob, conjugation transfer, competence

Әдебиет тізімі

Harwood C.R., Mouillon J.M., Pohl S., Arnau J. // FEMS Microbiol. Rev. 2018. V. 42. P. 721–738. https://doi.org/10.1093/femsre/fuy028
Burton A.T., Kearns D.B. // J. Bacteriol. 2020. V. 202. № 18. P. e00290-20. https://doi.org/10.1128/JB.00290-20
Singh P.K., Ramachandran G., Duran-Alcalde L., Alonso C., Wu L.J., Meijer W.J. // Environ. Microbiol. 2012. V. 14. № 10. P. 2812–2825. https://doi.org/10.1111/j.1462-2920.2012.02819x
Jeong D.E., Kim M.S., Kim H.R., Choi S.K. // Front. Microbiol. 2022. V. 13. P. 802040. https://doi.org/10.3389/fmicb.2022.802040
Brophy J.A.N., Triassi A.J., Adams B.L. // Nat. Microbiol. 2018. V. 3. P. 1043–1053. https://doi.org/10.1038/s41564-018-0216-5
Gurinovich A.S., Titok M.A. // Microbiology. 2022. V. 91. № 4. P. 395–408. https://doi.org/10.1134/S002626172230018X
Titok M.A., Chapuis J., Selezneva Y.V., Lagodich A.V., Prokulevich V.A., Ehrlich S.D., Jannière L. // Plasmid. 2003. V. 49. № 1. P. 53–62. https://doi.org/10.1016/s0147-619x(02)00109-9
Gurinovich A.S., Titok M.A. // Microbiology. 2020. V. 89. № 6. P. 660–669. https://doi.org/10.1134/S0026261720060065
Shatalin K.Y., Neyfakh A.A. // FEMS Microbiology Letters. 2005. V. 245. № 2. P. 315–319. https://doi.org/10.1016/j.femsle.2005.03.029
Harwood C.R., Cutting S.M. Molecular Biological Methods for Bacillus. / Ed. C.R. Harwood and S.M. Cutting Chichester, New York: Wiley, 1990. 581 p.
Anagnostopoulos C., Spizizen J. // J. Bacteriol. 1961. V. 81. № 5. P. 741–746. https://doi.org/10.1128/jb.81.5.741-746.1961
te Riele H., Michel B., Ehrlich S.D. // Proc. Natl. Acad. Sci. USA. 1986. V. 83. P. 2541–2545. https://doi.org/10.1073/pnas.83.8.2541
Sambrook J., Fritsch E., Maniatis T. Molecular Cloning: a Laboratory Manual, 2nd Ed. N.Y.: Cold Spring Harbor Laboratory Press, 1989. 468 p.
Poluektova E.U., Fedorina E.A., Lotareva O.V., Prozorov A.A. // Plasmid. 2004. V. 52. P. 212–217. https://doi.org/10.1016/j.plasmid.2004.07.001
Fujita Y, Satomura T, Tojo S, Hirooka K. // J. Bacteriol. 2014. V. 196. P. 3793–3806. https://doi.org/10.1128/JB.02055-14
Hoa T.T., Tortosa P., Albano M., Dubnau D. // Mol. Microbiol. 2002. V. 43. № 1. P. 15–26. https://doi.org/10.1046/j.1365-2958.2002.02727.x
Smits W.K., Grossman A.D. // PLoS Genet. 2010. V. 11. № 6. P. e1001207. https://doi.org/10.1371/journal.pgen.1001207