Мақаланы E-mail арқылы жіберу A STUDY OF ATROPINE INFLUENCE ON ACHE AND BCHE GENES EXPRESSION IN RATS EXPOSED TO MALATHION POISONING
- Авторлар: Babkin A.V.1, Berdinskih I.S.1, Osechkina N.S.2, Nazarov G.V.1, Yudin M.A.1, Bykov V.N.1
-
Мекемелер:
- Scientific Research Test Institute of Military Medicine of Medical Military Academy named after S.M. Kirov (The Russian Federation Department of Defense)
- Federal State Scientific Institution «Institute of Toxicology» Federal Medico-Biological Agency of Russia
- Шығарылым: № 6 (2015)
- Беттер: 22-26
- Бөлім: Articles
- ##submission.datePublished##: 04.12.2015
- URL: https://gynecology.orscience.ru/0869-7922/article/view/641358
- DOI: https://doi.org/10.15690/vramn521
- ID: 641358
Дәйексөз келтіру
Толық мәтін
Аннотация
Levels of expression of Ache and Bche genes in tissue samples were investigated in rats exposed to malathion in dose of 1 LD50 and following atropine treatment. Malathion poisoning causes statistically significant decrease in levels of expression of Ache and Bche genes in brain and liver. The decrease of expression of these genes was greater than 50% and remained at this level during 14 days. It was shown that atropine treatment prevents decrease in Ache gene expression in brain. These findings suggest the possibility of gene expression level assessment in brain during the screening of promising treatments for OP poisoning.
Негізгі сөздер
Авторлар туралы
Aleksandr Babkin
Scientific Research Test Institute of Military Medicine of Medical Military Academy named after S.M. Kirov (The Russian Federation Department of Defense)
Хат алмасуға жауапты Автор.
Email: noemail@neicon.ru
Научный сотрудник НИИИ ВМ ВМедА им. С.М. Кирова Министерства обороны Российской Федерации, 195043, г. Санкт-Петербург, Российская Федерация
e-mail: babkinnik@yandex.ru
РесейIrina Berdinskih
Scientific Research Test Institute of Military Medicine of Medical Military Academy named after S.M. Kirov (The Russian Federation Department of Defense)
Email: noemail@neicon.ru
Научный сотрудник НИИИ ВМ ВМедА им. С.М. Кирова Министерства обороны Российской Федерации, 195043, г. Санкт-Петербург, Российская Федерация
e-mail: irina_berdinskih@mail.ru
РесейNatalya Osechkina
Federal State Scientific Institution «Institute of Toxicology» Federal Medico-Biological Agency of Russia
Email: noemail@neicon.ru
Научный сотрудник ФГУН «Институт Токсикологии» ФМБА России, 192019, г. Санкт-Петербург, Российская Федерация
e-mail: dunats@rambler.ru
РесейGeorgiy Nazarov
Scientific Research Test Institute of Military Medicine of Medical Military Academy named after S.M. Kirov (The Russian Federation Department of Defense)
Email: noemail@neicon.ru
Доктор химических наук, доцент, начальник отдела НИИИ ВМ ВМедА им. С.М. Кирова Министерства обороны Российской Федерации, 195043, г. Санкт-Петербург, Российская Федерация
e-mail: denis-100@list.ru
РесейMikhail Yudin
Scientific Research Test Institute of Military Medicine of Medical Military Academy named after S.M. Kirov (The Russian Federation Department of Defense)
Email: noemail@neicon.ru
Кандидат медицинских наук, доцент, заместитель начальника отдела НИИИ (ВМ) ВМедА им. С.М. Кирова, 195043, г. Санкт-Петербург, Российская Федерация
e-mail: mikhail.judin@gmail.com
РесейVladimir Bykov
Scientific Research Test Institute of Military Medicine of Medical Military Academy named after S.M. Kirov (The Russian Federation Department of Defense)
Email: noemail@neicon.ru
Доктор медицинских наук, профессор, начальник НИИЦ (МБЗ) НИИИ (ВМ) ВМедА им. С.М. Кирова, 195043, г. Санкт-Петербург, Российская Федерация
e-mail: bykov_imm@yahoo.com
РесейӘдебиет тізімі
- Shilov V.V. et al. Study of the effectiveness of drugs in experimental models of poisoning neuropathy in malathion. //Occupational medicine and industrial ecology. 2013; 8: 13-18 (in Russian).
- Aurbek N. Suitability of human butyrylcholinesterase as therapeutic marker and pseudocatalytic scavenger inorganophosphate poisoning: A kinetic analysis. //Toxicology. 2009; 259: 133-139.
- Naik R.S. et al. Development and validation of a simple assay for the determination of cholinesterase activity in whole blood of laboratory animals.// J. Appl. Toxicol. 2013; 33 (4): 290-300.
- Bahar F.G. et al. Species difference of esterase expression and hydrolase activity in plasma.// J. Pharm. Sci. 2012; 101 (10): 3979-3988.
- Bajgar J. et al. Changes of cholinesterase activities in the rat blood and brain after sarin intoxication pretreated with butyrylcholinesterase. Drug. Chem. Toxicol. 2007; 30 (4): 351-3
- Babkin A.V. et al. Association of Bche gene polymorphic variants with activity of rat butyrylcholinesterase after exposure to malathion.// Toxicological Review. 2014; 5: 16-20 (in Russian).
- Lopez-Granero C. et al. Chlorpyrifos-, diisopropylphosphorofluoridate-, and parathion-induced behavioral and oxidative stress effects: are they mediated by analogous mechanisms of action. // Toxicol. Sci. 2013; 131 (1): 206-216.
- Xing, H. et al. Alterations in mRNA expression of acetylcholinesterase in brain and muscle of common carp exposed to atrazine and chlorpyrifos. //Ecotoxicol. Environ. Saf. 2010; 73 (7): 1666-16
- Mironov A.N. Guidelines for pre-clinical trials of drugs.Part Moscow. 2012 (in Russian).
- Osechkina N.S. et al. The expression’s influence and polymorphism of genes, which encode gaba-receptors, on heft ethanol influence in laboratory rats. // Toxicological Review. 2014; 6: 22-28 (in Russian).
- Rhee J. S. et al. Effect of pharmaceuticals exposure on acetylcholinesterase (AchE) activity and on the expression of AchE gene in the monogonont rotifer Brachionuskoreanus. //Comp. Biochem. Physiol. C. Toxicol. Pharmacol. 2013; 158 (4): 216-224.
- Zhen H. Toxic effects of HgCl2 on activities of SOD, AchE and relative expression of SOD, AChE, CYP1A1 of zebrafish. //Ecotoxicology. 2014; 23 (10): 1842-1845.
- Charoenying T. et al. Effects of paraoxon on neuronal and lymphocytic cholinergic systems.//Toxicol. Pharmacol. 2011; 31 (1): 119-128.
Қосымша файлдар
