Morphological changes in the liver of experimental animals in the early stages after correction of exposure to high doses of paracetamol

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Abstract

Introduction. Acetaminophen exhibits hepatotoxicity in overdose, which is the leading cause of drug-induced liver failure. The aim of the research was to study early morphological changes in the liver of experimental animals under the influence of high doses of paracetamol and after correction with hepatoprotectors.

Materials and methods. The corrective effect of various drugs — ademetionine, ethylmethylhydroxypyridine succinate and oxymethyluracil after exposure to high doses of acetaminophen was studied. Acetaminophen was administered to experimental rats once intragastrically at a dose of 1 g/kg, animals received hepatoprotectors twice (after 1 and 24 hours) or four times (after 1, 24, 48, 72 hours) after the toxicant. Morphological studies of the liver of experimental animals were carried out.

Results. Pathomorphological changes were revealed on rat liver preparations after exposure to paracetamol, the most pronounced after 72 hours of exposure. Oxymethyluracil has a protective effect on the structure of the liver during acute exposure to high doses of paracetamol, comparable to the well-known drugs “Heptor” and “Mexidol”. In addition, the protective effect of oxymethyluracil appears earlier.

Limitations. Morphological studies of the liver of 90 white male rats were carried out for studying the corrective action of hepatoprotectors after acute exposure to high doses of acetaminophen and after double (after 1 and 24 hours) or quadruple (after 1, 24, 48, 72 hours) administration of ademethionine, ethylmethylhydroxypyridine succinate and oxymethyluracil, which allowed to determine an effective means to reduce the toxic effect of paracetamol on the liver.

Conclusion. The data obtained may have a certain practical value in terms of the prospective use of oxymethyluracil as an additional effective agent for reducing the toxic effect of paracetamol.

About the authors

G. V. Timasheva

«Ufa research institute of occupational health and human ecology»

Author for correspondence.
Email: gulnara-vt60@yandex.ru

Candidate of Biological Sciences, Leading Researcher of the Department of Toxicology and Genetics with an experimental clinic of laboratory animals Ufa Research Institute of Occupational Health and Human Ecology, 450106, Ufa, Russian Federation.

e-mail: gulnara-vt60@yandex.ru 

Russian Federation

Samat Sagadatovich Baygildin

«Ufa research institute of occupational health and human ecology»

Email: baigildin.samat@yandex.ru

Младший научный сотрудник отдела токсикологии и генетики с экспериментальной клиникой лабораторных животных ФБУН «Уфимский НИИ медицины труда и экологии человека», 450106, Уфа.

e-mail: baigildin.samat@yandex.ru

Russian Federation

Ahat Barievich Bakirov

«Ufa research institute of occupational health and human ecology»; Bashkirian State Medical University of the Russian Health Ministry

Email: bakirov@anrb.ru

Доктор медицинских наук, профессор, директор ФБУН «Уфимский НИИ медицины труда и экологии человека», 450106, Уфа, заведующий кафедрой терапии и профессиональных болезней с курсом ИДПО БГМУ, 450008, Уфа.

e-mail: bakirov@anrb.ru 

Russian Federation

Elvira Faridovna Repina

«Ufa research institute of occupational health and human ecology»

Email: e.f.repina@bk.ru

Кандидат медицинских наук, старший научный сотрудник отдела токсикологии и генетики с экспериментальной клиникой лабораторных животных ФБУН «Уфимский НИИ медицины труда и экологии человека», 450106, Уфа.

e-mail: e.f.repina@bk.ru 

Russian Federation

Denis Olegovich Karimov

«Ufa research institute of occupational health and human ecology»

Email: karimovdo@gmail.com

Кандидат медицинских наук, заведующий отделом токсикологии и генетики с экспериментальной клиникой лабораторных животных ФБУН «Уфимский НИИ медицины труда и экологии человека», 450106, Уфа.

e-mail: karimovdo@gmail.com

Russian Federation

Nadezhda Yurevna Khusnutdinova

«Ufa research institute of occupational health and human ecology»

Email: h-n-yu@yandex.ru

Научный сотрудник отдела токсикологии и генетики с экспериментальной клиникой лабораторных животных ФБУН «Уфимский НИИ медицины труда и экологии человека», 450106, Уфа.

e-mail: h-n-yu@yandex.ru 

Russian Federation

Guzel Fanisovna Mukhammadieva

«Ufa research institute of occupational health and human ecology»

Email: ufniimt@mail.ru

Кандидат биологических наук, старший научный сотрудник отдела токсикологии и генетики с экспериментальной клиникой лабораторных животных ФБУН «Уфимский НИИ медицины труда и экологии человека», 450106, Уфа.

e-mail: ufniimt@mail.ru 

Russian Federation

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Copyright (c) 2022 Timasheva G.V., Baygildin S.S., Bakirov A.B., Repina E.F., Karimov D.O., Khusnutdinova N.Y., Mukhammadieva G.F.


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