Response of spring barley to UV and γ-irradiation

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Abstract

In two vegetation experiments on barley plants, the effect of separate and combined UV-B, UV-A and γ-irradiation in different doses was studied for the varieties “Zazersky 85” and “Vladimir. The plants were assessed for flavonoid content, photosynthetic pigments, morphology parameters and yield. The daily dose of chronic UV-B-irradiation was 9 kJ/m2, chronic UV-A – from 7.2 to 72 kJ/m2, single γ-irradiation 2 and 4 Gy. It has been shown that spring barley varieties “Zazersky 85” and “Vladimir” differ in their sensitivity to chronic UV-B irradiation. When predicting the negative impact of UV-B radiation on plants, it is necessary to take into account the existing level of UVA radiation, since their effect is not additive. Stimulation of biomass growth under chronic UV irradiation may be accompanied by suppression of plant productivity. The response of barley plants to γ-irradiation can be significantly dependent on the level of UV-(A+B) radiation. In general, the dose-dependent effect of chronic UV and γ-irradiation can be considered as a transition from austress to distress and its irreversible disruption. The data from this study may be relevant for programs to develop new varieties of spring barley that are resistant to elevated levels of solar UV radiation.

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About the authors

Oksana A. Guseva

Russian Institute of Radiology and Agroecology of National Research Centre “Kurchatov Institute”

Author for correspondence.
Email: gusevaoks65@yandex.ru
ORCID iD: 0000-0002-8814-6324
Russian Federation, Obninsk

Pavel N. Tsygvintsev

Russian Institute of Radiology and Agroecology of National Research Centre “Kurchatov Institute”

Email: paul-gomel@mail.ru
ORCID iD: 0000-0003-0214-7447
Russian Federation, Obninsk

Alexander N. Pavlov

Russian Institute of Radiology and Agroecology of National Research Centre “Kurchatov Institute”

Email: 49434@mail.ru
ORCID iD: 0000-0001-7714-2419
Russian Federation, Obninsk

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2. Fig. 1. Appearance of barley plants of the Zazersky 85 variety in the tillering phase under chronic UV irradiation. From left to right the study groups are presented: 1 — control, 1-2, 1-1, 1-6, 1-5, 1-4, 1-3).

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3. Fig. 2. Appearance of the main ear of barley variety “Vladimir” when plants are irradiated with UV-B and combined UV (A+B), where 1 – 2-control; 2 – group 2-3; 3 – group 2-11; 4 – group 2-6).

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4. Fig. 3. Content of flavonoids in barley leaves under chronic UV-irradiation) Note. The data for the barley variety “Zazerskiy 85” are shown in purple, and for the barley variety “Vladimir” in green. Statistically significant difference from the control: * р < 0.05; ** р < 0.01.

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