Influence of separate and combined action of γ-radiation and lead nitrate on germination, antioxidant status and cytogenetic indicators of spring barley seedlings

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Рұқсат ақылы немесе тек жазылушылар үшін

Аннотация

Exposure of seeds to a dose of γ-radiation of 20 Gy and a concentration of 2 mg/ml lead significantly modifies the oxidative status of barley plants. Lead at the concentration studied is capable of suppressing the development of seedlings, but is inferior to γ-radiation in its ability to induce cytogenetic abnormalities in the root meristem. Lead toxicity affects roots much more than shoots because roots interact directly with the lead solution. Preliminary seeds γ-irradiation modifies the oxidative status of plants, which reduces the negative effect of lead on seed germination and mitotic activity of cells. The combined action of lead and ionizing radiation does not lead to increased oxidative stress and an increase in the frequency of cytogenetic abnormalities compared to their separate action.

Толық мәтін

Рұқсат жабық

Авторлар туралы

Stanislav Geras’kin

National Research Center “Kurchatov Institute” – RIRAE

Хат алмасуға жауапты Автор.
Email: stgeraskin@gmail.com
ORCID iD: 0000-0001-9978-3049
Ресей, Obninsk

Alexander Prazyan

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
Ресей, Obninsk

Denis Vasiliev

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0003-0463-0029
Ресей, Obninsk

Sofia Bitarishvili

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0002-3623-7128
Ресей, Obninsk

Alena Smirnova

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0009-0006-1263-9188
Ресей, Obninsk

Ekaterina Shesterikova

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0001-6737-5753
Ресей, Obninsk

Anastasiya Khanova

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0003-1171-0844
Ресей, Obninsk

Ivan Pishenin

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0002-2633-9251
Ресей, Obninsk

Elizaveta Kazakova

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0002-2975-5891
Ресей, Obninsk

Ekaterina Kvichanskaia

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0001-7519-9550
Ресей, Obninsk

Mariya Lychenkova

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0001-6148-2021
Ресей, Obninsk

Darya Babina

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0003-2544-9667
Ресей, Obninsk

Marina Korol

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0003-0911-2650
Ресей, Obninsk

Yana Blinova

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
Ресей, Obninsk

Mikhail Podlutskii

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0002-7890-0469
Ресей, Obninsk

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2. Fig. 1. Conditions for barley germination using the roll method for biochemical studies (A) and in Petri dishes for cytogenetic analysis (B).

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3. Fig. 2. Catalase activity in shoots (A) and roots (B) of 7-day-old barley seedlings after separate and combined effects of γ-radiation and lead. * Significant relative to control, p < 0.05, Mann–Whitney U-test.

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4. Fig. 3. Activity of ascorbate peroxidase in shoots (A) and roots (B) of 7-day-old barley seedlings after separate and combined effects of γ-radiation and lead. * Significant relative to control, p < 0.05, Mann–Whitney U-test. + Significant relative to the combined effect of factors, p < 0.05, Mann–Whitney U-test.

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5. Fig. 4. Activity of guaiacol peroxidase in shoots (A) and roots (B) of 7-day-old barley seedlings after separate and combined effects of γ-radiation and lead.

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6. Fig. 5. Concentrations of ascorbic acid in shoots (A) and roots (B) of 7-day-old barley seedlings after separate and combined effects of γ-radiation and lead. * Significant relative to control, p < 0.05, Mann–Whitney U-test. + Significant relative to irradiated plants, p < 0.05, Mann–Whitney U-test.

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7. Fig. 6. Concentrations of oxidized glutathione in shoots (A) and roots (B) of 7-day-old barley seedlings after separate and combined effects of γ-radiation and lead. ▼ Significant relative to irradiated plants, p < 0.05, Mann–Whitney U-test.

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8. Fig. 7. Concentrations of reduced glutathione in sprouts (A) and roots (B) of 7-day-old barley seedlings after separate and combined exposure to γ-radiation and lead. * Significant relative to control, p < 0.05, Mann–Whitney U-test. ▼ Significant relative to irradiated plants, p < 0.05, Mann–Whitney U-test.

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9. Fig. 8. Concentrations of MDA in shoots (A) and roots (B) of 7-day-old barley seedlings after separate and combined effects of γ-radiation and lead. * Significant relative to control, p < 0.05, Mann–Whitney U-test. ▼ Significant relative to irradiated plants, p < 0.05, Mann–Whitney U-test.

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10. Fig. 9. Seed germination (A) and mitotic index in cells of the root meristem of barley seedlings (B) on the 7th day of germination after separate and combined action of γ-radiation and lead salt. * Difference from control is statistically significant, p < 0.05.

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