Application of lactoferrin for the prevention and restoration of bone tissue in Wistar rats under conditions of hindlimb unloading

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The influence of gravitational unloading (antiorthostatic suspension) and subsequent recovery on the mineral density and mechanical properties of the femoral and tibial bones of Wistar rats was studied with oral administration of a biotechnological analog of human lactoferrin (200 mg/kg) derived from the milk of producer goats. Bone mineral density was determined by dual-energy X-ray absorptiometry, and strength and stiffness were assessed through three-point bending tests.

It was shown that gravitational unloading for 21 days led to a decrease in the mineral density of the tibial and femoral bones. The administration of lactoferrin did not significantly affect the mineral density or projected area of the studied bones. No statistically significant differences in mechanical stiffness were found between the experimental groups, but after readaptation, the ultimate strength was significantly higher in the groups that received lactoferrin. Thus, the obtained results may indicate the potential of lactoferrin preparations as prophylactic agents for maintaining bone strength. At the same time, maintaining bone mineral density under deficit-stimulating conditions requires consideration of alternative dosages and delivery methods of the drug.

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Sobre autores

K. Gordienko

Institute for Biomedical Problems of the Russian Academy of Sciences

Autor responsável pela correspondência
Email: k.vl.gordienko@gmail.com
Rússia, Moscow

N. Lukicheva

Institute for Biomedical Problems of the Russian Academy of Sciences

Email: k.vl.gordienko@gmail.com
Rússia, Moscow

A. Akhmetzyanova

Kazan (Volga region) Federal University

Email: k.vl.gordienko@gmail.com
Rússia, Kazan

A. Kolupaev

Institute of Gene Biology Russian Academy of Sciences

Email: k.vl.gordienko@gmail.com
Rússia, Moscow

O. Sachenkov

Kazan (Volga region) Federal University

Email: k.vl.gordienko@gmail.com
Rússia, Kazan

T. Baltina

Kazan (Volga region) Federal University

Email: k.vl.gordienko@gmail.com
Rússia, Kazan

E. Sadchikova

Institute of Gene Biology Russian Academy of Sciences

Email: k.vl.gordienko@gmail.com
Rússia, Moscow

G. Vassilieva

Institute for Biomedical Problems of the Russian Academy of Sciences

Email: k.vl.gordienko@gmail.com
Rússia, Moscow

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2. Fig. 1. Young's modulus. CON – control groups, HS – suspended groups, HSR – suspended groups with subsequent readaptation, PL – groups taking placebo, LF – groups taking lactoferrin.

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3. Fig. 2. Tensile strength. CON – control groups, HS – suspended groups, HSR – suspended groups with subsequent readaptation, PL – groups taking placebo, LF – groups taking lactoferrin.

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