Time-course Expression of Mechanosensitive Ion Channels in Rat Postural Muscle under Hindlimb Unloading

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Atony and atrophy of mammalian postural muscles can occur due to mechanical unloading (weightlessness, hypokinesia). There is reason to believe that calcium-permeable mechanosensitive channels may contribute to the development of muscle atrophy caused by mechanical unloading. The aim of the study was to assess time-course changes in the expression of key mechanosensitive channels in rat soleus muscle under conditions of mechanical unloading. Male Wistar rats were subjected to hindlimb suspension (HS) for 1, 3, 7 and 14 days. Expression of Piezo1, TRPC1, TRPC3, TRPC6, TRPM3, TRPM7 and TMEM63B mRNA was determined using PCR. Piezo1 protein content was assessed using Western blotting. Piezo1 mRNA expression transiently increased after 24 h of HS, but did not differ from the control after 3, 7 and 14 days of unloading. A decrease in Piezo1 protein content was observed after 3, 7 and 14 days of HS relative to the control. At the early stages of HS, there was a significant increase in the mRNA expression of TRPC3, TRPM3, TRPM7 and TMEM63B, while TRPC6 expression was reduced. The level of TRPC1 mRNA expression was increased only after 3 days of HS. Seven-day unloading did not cause changes in the mRNA expression of TRPC1, TRPC3, TRPM3 and TMEM63B but led to increased TRPM7 expression. After two weeks of HS in the soleus muscle, a decrease in the mRNA expression of TRPC1, TRPC6, TRPM3 and TMEM63B was observed. Thus, at an early stage of mechanical unloading (1 and 3 days), a transient increase in the mRNA expression of Piezo1, TRPC1, TRPC3 and TMEM63B is observed, then at a later stage of unloading (14 days), reduced expression of TRPC1, TRPC6, TRPM3, TMEM63B at the mRNA level and Piezo1 at the protein level is observed.

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N. Vilchinskaya

Institute of Biomedical Problems of the Russian Academy of Sciences

编辑信件的主要联系方式.
Email: tmirzoev@yandex.ru
俄罗斯联邦, Moscow

B. Shenkman

Institute of Biomedical Problems of the Russian Academy of Sciences

Email: tmirzoev@yandex.ru
俄罗斯联邦, Moscow

T. Mirzoev

Institute of Biomedical Problems of the Russian Academy of Sciences

Email: tmirzoev@yandex.ru
俄罗斯联邦, Moscow

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2. Fig. 1. Piezo1 mRNA expression level (a) and Piezo1 protein content (b) in rat m. soleus during functional unloading. C, control; HS1, HS3, HS7, HS14, 1-, 3-, 7-, 14-day hind limb suspension; *, significant difference from C (p < 0.05).

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3. Fig. 2. TRPC1 (a), TRPC3 (b) and TRPC6 (c) mRNA expression levels in rat m. soleus during functional unloading. C, control; HS1, HS3, HS7, HS14, 1-, 3-, 7-, 14-day hind limb suspension; *, significant difference from C (p < 0.05).

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4. Fig. 3. TMEM63B (a), TRPM3 (b) and TRPM7 (c) mRNA expression levels in rat m. soleus during functional unloading. C, control; HS1, HS3, HS7, HS14, 1-, 3-, 7-, 14-day hind limb suspension; *, significant difference from C (p < 0.05).

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