The Peculiarities of Neutrophils Migration Processes in the Flow System

Cover Page

Cite item

Full Text

Abstract

A flow system model simulating the hemodynamic process in capillaries was created to study neutrophil migration. It was found that the general patterns of neutrophil behavior (aggregate formation, formation of neutrophil tethers and migration of neutrophils along intercellular contacts of endothelial cells) are reproduced both in the control (system without chemoattractants) and in the experiment (system with low-molecular chemoattractants of bacteria), however, in the case of S. aureus and P. mirabilis using as a chemoattractant, the number of aggregates and neutrophil tethers increases statistically significantly (p < 0.05). Aggregates correspond to the swarming phenomenon in the transendothelial migration system and help limit the zone of bacterial damage. And neutrophil tethers cause a stop in hemodynamic movement and can cause either a transition to migration or be precursors to the formation of elongated neutrophil-derived structures with high antibacterial potential. Thus, all the observed phenomena contribute to the implementation of the protective function in case of bacteremia.

About the authors

S. N. Pleskova

Lobachevsky Nizhny Novgorod State University; Nizhny Novgorod State Technical University named after R.E. Alekseev

Email: pleskova@mail.ru
Nizhny Novgorod, Russia; Nizhny Novgorod, Russia

N. A. Bezrukov

Lobachevsky Nizhny Novgorod State University

Nizhny Novgorod, Russia

E. N. Gorshkova

Lobachevsky Nizhny Novgorod State University

Nizhny Novgorod, Russia

D. V. Novikov

Lobachevsky Nizhny Novgorod State University; Nizhny Novgorod Research Institute of Epidemiology and Microbiology named after Academician I.N. Blokhina

Nizhny Novgorod, Russia; Nizhny Novgorod, Russia

E. V. Otstavnova

Lobachevsky Nizhny Novgorod State University; Nizhny Novgorod State Technical University named after R.E. Alekseev

Nizhny Novgorod, Russia; Nizhny Novgorod, Russia

References

  1. Kolaczkowska E, Kubes P (2013) Neutrophil recruitment and function in health and inflammation. Nature reviews. Immunology 13: 159–175. https://doi.org/10.1038/nri3399
  2. Voisin M-B, Nourshargh S (2019) Neutrophil trafficking to lymphoid tissues: physiological and pathological implications. J Pathol 247: 662–671. https://doi.org/10.1002/path.5227
  3. Li Y, Wang W, Yang F, Xu Y, Feng C, Zhao Y (2019) The regulatory roles of neutrophils in adaptive immunity. Cell Commun Signal 17: 147. https://doi.org/10.1186/s12964-019-0471-y
  4. Ley K, Hoffman HM, Kubes P, Cassatella MA, Zychlinsky A, Hedrick CC, Catz SD (2018) Neutrophils: New insights and open questions. Sci Immunol 3: eaat4579. https://doi.org/10.1126/sciimmunol.aat4579
  5. Ley K, Laudanna C, Cybulsky MI, Nourshargh S (2007) Getting to the site of inflammation: the leukocyte adhesion cascade updated. Nat Rev Immunol 7: 678–689. https://doi.org/10.1038/nri2156
  6. Buffone A, Hammer DA, Kim SHJ, Anderson NR, Mochida A, Lee DH, Guin S (2023) Not All (Cells) Who Wander Are Lost: Upstream Migration as a Pervasive Mode of Amoeboid Cell Motility. Front Cell Dev Biol 11: 1291201. https://doi.org/10.3389/fcell.2023.1291201
  7. Pleskova SN, Bezrukov NA, Gorshkova EN, Bobyk SZ, Lazarenko EV (2024) A Study of EA.hy926 Endothelial Cells Using Atomic Force and Scanning Ion Conductance Microscopy. Cell Tiss Biol 18: 36–44. https://doi.org/10.1134/S1990519X24010073
  8. Pleskova SN, Bezrukov NA, Gorshkova EN, Bobyk SZ, Lazarenko EV (2023) Exploring the Process of Neutrophil Transendothelial Migration Using Scanning Ion-Conductance Microscopy. Cells 12: 1806. https://doi.org/10.3390/cells12131806
  9. Cugno A, Marki A, Ley K (2021) Biomechanics of Neutrophil Tethers. Life (Basel) 11: 515. https://doi.org/10.3390/life11060515
  10. Lammermann T, Afonso PV, Angermann BR, Wang JM, Kastenmüller W, Parent CA, Germain RN (2013) Neutrophil swarms require LTB4 and integrins at sites of cell death in vivo. Nature 498: 371–375. https://doi.org/10.1038/nature12175
  11. Lee EKS, Gillrie MR, Li L, Arnason JW, Kim JH, Babes L, Lou Y, Sanati-Nezhad A, Kyei SK, Kelly MM, Mody CH, Ho M, Yipp BG (2018) Leukotriene B4-Mediated Neutrophil Recruitment Causes Pulmonary Capillaritis during Lethal Fungal Sepsis. Cell Host Microbe 23: 121–133.e4. https://doi.org/10.1016/j.chom.2017.11.009
  12. Pleskova SN, Kriukov RN, Gorshkova EN, Boryakov AV (2019) Characteristics of quantum dots phagocytosis by neutrophil granulocytes. Heliyon 5: e01439. https://doi.org/10.1016/j.heliyon.2019.e01439
  13. Yang P, Li Y, Xie Y, Liu Y (2019) Different Faces for Different Places: Heterogeneity of Neutrophil Phenotype and Function. J Immunol Res 2019: 8016254. https://doi.org/10.1155/2019/8016254
  14. Kienle K, Glaser KM, Eickhoff S, Mihlan M, Knopper K, Reategui E, Epple MW, Gunzer M, Baumeister R, Tarrant TK, Germain RN, Irimia D, Kastenmuller W, Lammermann T (2021) Neutrophils self-limit swarming to contain bacterial growth in vivo. Science 372: eabe7729. https://doi.org/10.1126/science.abe7729
  15. Kunkel EJ, Dunne JL, Ley K (2000) Leukocyte Arrest During Cytokine-Dependent Inflammation In Vivo. J Immunol 164: 3301–3308. https://doi.org/10.4049/jimmunol.164.6.3301
  16. Girdhar G, Shao JY (2007) Simultaneous Tether Extraction from Endothelial Cells and Leukocytes: Observation, Mechanics, and Significance. Biophys J 93: 4041–4052. https://doi.org/10.1529/biophysj.107.109298
  17. Chen Y, Yao DK, Shao JY (2010) The Constitutive Equation for Membrane Tether Extraction. Ann Biomed Eng 38: 3756–3765. https://doi.org/10.1007/s10439-010-0117-0
  18. Marki A, Gutierrez E, Mikulski Z, Groisman A, Ley K (2016) Microfluidics-based side view flow chamber reveals tether-to-sling transition in rolling neutrophils. Sci Rep 6: 28870. https://doi.org/10.1038/srep28870

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2025 Russian Academy of Sciences