The blood leukocytes and platelets of little ground squirrels (Spermophilus pygmaeus Pall.) during hibernation and arousal

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Abstract

During hibernation, mammals periodically go through cold (torpor) and warm (awakening) phases. Previously, a sharp decrease in the levels of leukocytes and blood platelets in the torpid state was found, but the dynamics of their changes during arousal remains unknown. We have studied the content and composition of the circulating cells of the little ground squirrel during arousal. The number of all types of leukocytes and platelets in the torpid state is significantly reduced. Restoration of the level of leukocytes in the blood during arousal occurs in two phases: fast (Tb 10—20°C) and slow (Tb 20—37°C). Unlike other cell types, the content of neutrophils remains below euthermal condition after body temperature recovery. During arousal in the Tb range of 10—30°C, the level of platelets increases linearly, while the normalization of the P-LCR parameter does not occur.

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A. M. Dzhafarova

Dagestan State University

Email: klich-khan@mail.ru
Russian Federation, Makhachkala

Sh. I. Chalabov

Dagestan State University; I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences

Email: klich-khan@mail.ru
Russian Federation, Makhachkala; Saint Petersburg

N. K. Klichkhanov

Dagestan State University

Author for correspondence.
Email: klich-khan@mail.ru
Russian Federation, Makhachkala

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2. Fig. 1. Dynamics of changes in WBC (a), LYM (b), NEUT (c) and MXD (d) in gopher blood during winter hibernation and induced awakening (Me [Q1: Q3]), minimum and maximum sample value; p<0.05 vs: * - control, + - hibernation, # - initial stages of awakening Tb10°C). K - control, C - torpid state. The arrow indicates body temperature during awakening

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3. Fig. 2. Regression analysis of the effect of gopher body temperature on blood WBCs

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4. Fig. 3. Dynamics of PLT (a), MPV (b), PCT (c) and P-LCR (d) changes in gopher blood during winter hibernation and induced awakening (Me [Q1: Q3], minimum and maximum sample values in the diagrams; Me [Q1: Q3]; p<0.05 relative to * - control, + - hibernation, # - initial stages of Tb10°C awakening). K - control, C - torpid state. The arrow indicates body temperature during awakening

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5. Fig. 4. Regression analysis of the effect of gopher body temperature on blood PLT

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6. Fig. 5. Dynamics of PDW changes in gopher blood during winter hibernation and induced awakening (Me [Q1: Q3]), minimum and maximum sample values; p<0.05 relative to * - control, + - hibernation, # - initial stages of Tb10°C awakening. K - control, C - torpid state. The arrow indicates body temperature during awakening

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7. Fig. 6. Correlation between WBC and PLT in blood of gophers during winter hibernation and induced awakening. K - control, C - torpid state

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