Investigation of the Potential Mechanism of Compound Dragon's Blood Capsule against Myocardial Ischemia Based on Network Pharmacology


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Abstract

Background:Dragon's blood is widely consumed in China, Vietnam and Laos to promote blood circulation. A Compound Dragon's blood capsule (CDC) is a patented medicine composed of dragon’s blood, notoginseng, and borneol. This combination is purported to stabilize coronary heart disease and myocardial ischemia. However, the possible mechanisms and the characterization of its drug targets’ relevance at the systemic level remain unclear.

Aim:The present study aims to reveal the potential mechanisms of CDC’s anti-myocardial ischemia effect

Materials and Methods:The potential mechanisms were investigated by network pharmacology and qRT-PCR was used to verify the expression levels of key genes of PI3k-Akt pathway.

Results:S1PR2 and AGTR1 were the common targets, which involved 6 biological processes annotated by KEGG and GO analysis. The qRT-PCR results showed a remarkable increase in the expression of Pi3k, Pdk1, Akt, Mdm2, Bcl2, and mTOR. Results also showed a decline in the expression of P53 and Casp3 after CDC intervention.

Conclusion:CDC has a significant anti-myocardial ischemia effect through the PI3k/Akt pathway, which demonstrates that CDC is a suitable adjuvant to treat CHD and provides a theoretical basis for its further clinical application.

About the authors

Xin Su

Chinese Academy of Medical Sciences, Yunnan Branch, Institute of Medicinal Plant Development

Email: info@benthamscience.net

Hongwei Xue

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College

Email: info@benthamscience.net

Yang Lou

Chinese Academy of Medical Sciences, Peking Union Medical College, Institute of Medicinal Plant Development

Email: info@benthamscience.net

Xinkai Lv

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College

Email: info@benthamscience.net

Xiao Mi

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College

Email: info@benthamscience.net

Juan Lu

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College

Author for correspondence.
Email: info@benthamscience.net

Xi Chen

Chinese Academy of Medical Sciences, Peking Union Medical College, Institute of Medicinal Plant Development

Author for correspondence.
Email: info@benthamscience.net

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