Human Adipose-derived Stem Cells Upregulate IGF-1 and Alleviate Osteoarthritis in a Two-stage Rabbit Osteoarthritis Model

  • Autores: Wang J.1, Su S.2, Dong C.3, Fan Q.4, Sun J.5, Liang S.6, Qin Z.2, Ma C.7, Jin J.8, Zhu H.9, Jiang T.10, Xu J.11
  • Afiliações:
    1. Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University
    2. School of Basic Medicine and Life Sciences, Hainan Medical University
    3. Department of Anatomy, Medical College of Nantong University
    4. Orthopedics Department, Qingdao Jimo District People's Hospital
    5. Neurosurgery Department,, Qingdao Jimo District People's Hospital
    6. , Zhongke Comprehensive Medical Transformation Center Research Institute (Hainan) Co., Ltd
    7. The Second Clinical College,, Hainan Medical University
    8. Department of Biochemistry, School of Basic Medicine and Life Sciences,, Hainan Medical University
    9. Orthopedics Department, Tianjin Hospital
    10. Engineering Research Center for Hainan Bio-Smart Materials and Bio-Medical Devices, Key Laboratory of Emergency and Trauma, Ministry of Education, Key Laboratory of Hainan Functional Materials and Molecular Imaging, College of Emergency and Trauma,, Hainan Medical University
    11. Stem Cell Center, Shanghai East Hospital, School of Medicine, Tongji University
  • Edição: Volume 19, Nº 11 (2024)
  • Páginas: 1472-1483
  • Seção: Medicine
  • URL: https://gynecology.orscience.ru/1574-888X/article/view/645589
  • DOI: https://doi.org/10.2174/011574888X274359231122064109
  • ID: 645589

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Resumo

Objective:In recent times, it has been recognized that mesenchymal stem cells (MSCs) possess the capability to address osteoarthritis (OA). The objective of this research was to examine the impact of injecting human adipose-derived stem cells (hADSCs) into a novel rabbit osteoarthritis model with dual damage.

Methods:The OA model was established surgically first by medial collateral ligament and anterior cruciate ligament transection and medial meniscectomy, then by articular cartilage full-thickness defect. Enhanced Green Fluorescence Protein expressing lentivirus FG12 was used to label hADSCs, which were then injected into the knee joints. Every single rabbit was sacrificed after 4 and 8 weeks following the surgical procedure. Macroscopic examination, immunohistochemistry staining, magnetic resonance imaging, qRT-PCR, and ELISA analysis were utilized for the assessments.

Results:After 4 and 8 weeks, the injection of hADSCs resulted in reduced cartilage loss, minimal fissures and cracks, and a decrease in the volume of joint effusion and cartilage defect as measured by MRI. Moreover, the application of ELISA and qRT-PCR techniques revealed that the administration of hADSCs resulted in an elevation in the IGF-1 concentration.

Conclusions:Based on our findings, it can be inferred that the transplantation of hADSCs facilitates the healing of articular cartilage in the osteoarthritis model of rabbits with double damage. The upregulated IGF-1 may play a crucial part in the process of cartilage repair using hADSCs. The use of hADSC transplantation could potentially be appropriate for clinical implementation in managing osteoarthritis.

Sobre autores

Juan Wang

Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University

Autor responsável pela correspondência
Email: info@benthamscience.net

Shibo Su

School of Basic Medicine and Life Sciences, Hainan Medical University

Email: info@benthamscience.net

Chuanming Dong

Department of Anatomy, Medical College of Nantong University

Email: info@benthamscience.net

Qiang Fan

Orthopedics Department, Qingdao Jimo District People's Hospital

Email: info@benthamscience.net

Jishu Sun

Neurosurgery Department,, Qingdao Jimo District People's Hospital

Email: info@benthamscience.net

Siqiang Liang

, Zhongke Comprehensive Medical Transformation Center Research Institute (Hainan) Co., Ltd

Email: info@benthamscience.net

Zuhuo Qin

School of Basic Medicine and Life Sciences, Hainan Medical University

Email: info@benthamscience.net

Chuqing Ma

The Second Clinical College,, Hainan Medical University

Email: info@benthamscience.net

Jianfeng Jin

Department of Biochemistry, School of Basic Medicine and Life Sciences,, Hainan Medical University

Email: info@benthamscience.net

Hongwen Zhu

Orthopedics Department, Tianjin Hospital

Autor responsável pela correspondência
Email: info@benthamscience.net

Tongmeng Jiang

Engineering Research Center for Hainan Bio-Smart Materials and Bio-Medical Devices, Key Laboratory of Emergency and Trauma, Ministry of Education, Key Laboratory of Hainan Functional Materials and Molecular Imaging, College of Emergency and Trauma,, Hainan Medical University

Autor responsável pela correspondência
Email: info@benthamscience.net

Jun Xu

Stem Cell Center, Shanghai East Hospital, School of Medicine, Tongji University

Autor responsável pela correspondência
Email: info@benthamscience.net

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