Cinnamic Acid Ameliorates Acetic Acid-induced Inflammatory Response through Inhibition of TLR-4 in Colitis Rat Model


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Background:Cinnamic acid, an active compound in cinnamon spp., has anti-inflammatory and antioxidant characteristics and is favorable in managing inflammatory bowel diseases.

background:Background: Cinnamic acid is a chemical with anti-inflammatory and antioxidant characteristics, thus might help to manage inflammatory bowel disease.

Objective:Evaluate cinnamic acid's effects on colitis in rats.

Methods:To induce colitis in experimental rats, excluding the sham group, a 4% intrarectal solu-tion of acetic acid was administered. The rats were then given oral doses of cinnamic acid at 30, 45, and 90 mg/kg for two days. The animals were assessed for macroscopic and microscopic changes, and the levels of inflammatory mediators such as tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and myeloperoxidase (MPO) were measured using Eliza kits. Additionally, real-time PCR was performed to examine the gene level of toll-like receptor 4 (TLR-4) in the colon.

Results:Effective reduction of inflammation in acetic acid-induced colitis was achieved through Cinnamic acid administration at doses of 45 and 90 mg/kg. The decrease was achieved by inhibiting the activities of TNF-α, IL-6, and MPO while downregulating the expression of TLR-4. It is important to note that macroscopic and microscopic evaluations were significant in determining the effectiveness of cinnamic acid in reducing inflammation.

Conclusion:Downregulation of inflammatory cytokines and TLR-4 expression may contribute to cinnamic acid's anti-inflammatory effect.

作者简介

Pardis Gharazi

Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University

Email: info@benthamscience.net

Mahban Rahimifard

Department of Toxicology and Pharmacology, Faculty of Pharmacy, And Toxicology & Diseases Group (TDG), Pharmaceutical Sciences Research Center (PSRC), Tehran University of Medical Sciences

Email: info@benthamscience.net

Maryam Baeeri

Department of Toxicology and Pharmacology, Faculty of Pharmacy, And Toxicology & Diseases Group (TDG), Pharmaceutical Sciences Research Center (PSRC), Tehran University of Medical Sciences

Email: info@benthamscience.net

Ali Abdollahi

Department of Pathology, Imam Hospital, Tehran University of Medical Sciences

Email: info@benthamscience.net

Mohammad Abdollahi

Department of Toxicology and Pharmacology, Faculty of Pharmacy, And Toxicology & Diseases Group (TDG), Pharmaceutical Sciences Research Center (PSRC), Tehran University of Medical Sciences

Email: info@benthamscience.net

Amirhossein Niknejad

Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University

Email: info@benthamscience.net

Danial Khayatan

Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University

Email: info@benthamscience.net

Mohammad Farzaei

Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences

Email: info@benthamscience.net

Amir Abdolghaffari

Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University

编辑信件的主要联系方式.
Email: info@benthamscience.net

Zahra Rezaei

Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran Medical Sciences, Islamic Azad University

Email: info@benthamscience.net

Saeideh Momtaz

Medicinal Plants Research Center, Institute of Medicinal Plants

编辑信件的主要联系方式.
Email: info@benthamscience.net

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