Antimalarial Effects of Nano Chloroquine Loaded Curcumin In vivo


Дәйексөз келтіру

Толық мәтін

Аннотация

Background:Malaria is still the deadliest parasitic disease caused by Plasmodium spp. Due to drug resistance and their unpleasant side effects, of conventional researchers are enormously seeking to achieve antimalarial drugs with more curative effective, less toxic and cost-affordable drugs using more advanced technology such as nanodrugs

Purpose:The present study aimed to examine the antimalarial effects of a novel synthesized nonochloroquine-loaded curcumin relying on dendrimer G2 in susceptible mice.

Methods:Antimalarial activity and toxicity of the nanocomposite were examined on BALB/C mice with microscopy, checking RBCs morphology and related enzymatic activity rate.

Results:The maximum inhibitory effect of the nanocomposite was seen at 10 mg/kg, killing 98% of P. berghei compared to sole chloroquine, whereas ED50 was reported at 5.5 mg/kg. The safety of the synthesized nanocomposite was confirmed with biochemical tests with no detrimental effects on mice. The sustainability and longevity of the nanodrug increased significantly with the NDC-CQ assay compared to the control groups

Conclusion:The study showed that nonochloroquine-loaded curcumin had a promising inhibitory effect on P. berghei growth in infected mice compared to standard drugs. However, further studies and clinical trials with large samples are recommended to study different aspects of using nanodrug.

Авторлар туралы

Taher Elmi

Department of Laboratory Science, School of Medicine, Islamic Azad University

Email: info@benthamscience.net

Fatemeh Tabatabaie

Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences

Хат алмасуға жауапты Автор.
Email: info@benthamscience.net

Mehdi Ardestani

Department of Radio Pharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences

Email: info@benthamscience.net

Abdolhossein Dalimi

Department of Parasitology, Faculty of Medical Sciences,, Tarbiat modares University

Email: info@benthamscience.net

Fatemeh Ghaffarifar

Department of Parasitology, Faculty of Medical Sciences, Tarbiat modares University

Email: info@benthamscience.net

Zahra Zamani

Biochemistry Department, Pasteur Institute of Iran

Email: info@benthamscience.net

Fatemeh Maleki

Department of Parasitology, School of Allied Medical Sciences, Iran University of Medical Sciences

Хат алмасуға жауапты Автор.
Email: info@benthamscience.net

Әдебиет тізімі

  1. Ahmadpour E, Foroutan-Rad M, Majidiani H, et al. Transfusion-transmitted Malaria: A systematic review and meta-analysis. Open Forum Infect Dis 2019; 6(7): ofz283. doi: 10.1093/ofid/ofz283 PMID: 31334300
  2. Elmi T, Ardestani MS, Hajialiani F, et al. Novel chloroquine loaded curcumin based anionic linear globular dendrimer G2:A metabolomics study on Plasmodium falciparum in vitro using 1H NMR spectroscopy. Parasitology 2021; 148(13): 1715-5. doi: 10.1017/S0031182021000457 PMID: 34425930
  3. Elmi T, Ardestani MS, Motevalian M, et al. Antiplasmodial effect of nano dendrimer G2 loaded with chloroquine in mice infected with plasmodium berghei. Acta Parasitol 2022; 67(1): 298-308. doi: 10.1007/s11686-021-00459-4 PMID: 34398379
  4. Bartoloni A, Zammarchi L. Clinical aspects of uncomplicated and severe malaria. Mediterr J Hematol Infect Dis 2012; 4(1): e2012026. doi: 10.4084/mjhid.2012.026 PMID: 22708041
  5. Talapko J, Škrlec I, Alebić T, Jukić M, Včev A. Malaria: The past and the present. Microorganisms 2019; 7(6): 179. doi: 10.3390/microorganisms7060179 PMID: 31234443
  6. Ibraheem ZO, Majid RA, Sidek HM, et al. In vitro antiplasmodium and chloroquine resistance reversal effects of andrographolide. Evid Based Complement Alternat Med 2019; 2019: 1-16. doi: 10.1155/2019/7967980 PMID: 31915453
  7. Mukherjee R, Patra M, Dutta D, Banik M, Basu T. Tetracycline-loaded calcium phosphate nanoparticle (Tet-CPNP): Rejuvenation of an obsolete antibiotic to further action. Biochim Biophys Acta 2016; 1860(9): 1929-41. doi: 10.1016/j.bbagen.2016.06.006
  8. Namazi H, Motamedi S, Namvari M. Synthesis of new functionalized citric acid-based dendrimers as nanocarrier agents for drug delivery. Bioimpacts 2011; 1(1): 63-9. doi: 10.5681/bi.2011.009 PMID: 23678409
  9. Abbasi E, Aval SF, Akbarzadeh A, et al. Dendrimers: Synthesis, applications, and properties. Nanoscale Res Lett 2014; 9(1): 247. doi: 10.1186/1556-276X-9-247 PMID: 24994950
  10. Lo ST, Kumar A, Hsieh JT, Sun X. Dendrimer nanoscaffolds for potential theranostics of prostate cancer with a focus on radiochemistry. Mol Pharm 2013; 10(3): 793-812. doi: 10.1021/mp3005325 PMID: 23294202
  11. Mehrizi TZ, Ardestani MS, Molla Hoseini MH, Khamesipour A, Mosaffa N, Ramezani A. Novel nano-sized chitosan amphotericin B formulation with considerable improvement against Leishmania major. Nanomedicine 2018; 13(24): 3129-47. doi: 10.2217/nnm-2018-0063 PMID: 30463469
  12. Haririan I, Alavidjeh MS, Khorramizadeh MR, Ardestani MS, Ghane ZZ, Namazi H. Anionic linear-globular dendrimer-cis-platinum (II) conjugates promote cytotoxicity in vitro against different cancer cell lines. Int J Nanomedicine 2010; 5(1): 63-75. doi: 10.2147/IJN.S8595 PMID: 20161988
  13. Alavidjeh MS, Haririan I, Khorramizadeh MR, Ghane ZZ, Ardestani MS, Namazi H. Anionic linear-globular dendrimers: Biocompatible hybrid materials with potential uses in nanomedicine. J Mater Sci Mater Med 2010; 21(4): 1121-33. doi: 10.1007/s10856-009-3978-8 PMID: 20082119
  14. Soniran OT, Idowu OA, Ajayi OL, Olubi IC. Comparative study on the effects of chloroquine and artesunate on histopathological damages caused by plasmodium berghei in four vital organs of infected albino mice. Malar Res Treat 2012; 2012: 1-7. doi: 10.1155/2012/960758 PMID: 22792509
  15. Memar B, Jamili S, Shahbazzadeh D, Bagheri KP. The first report on coagulation and phospholipase A2 activities of Persian Gulf lionfish, Pterois russelli, an Iranian venomous fish. Toxicon 2016; 113: 25-31. doi: 10.1016/j.toxicon.2016.02.004 PMID: 26853495
  16. Knight DJ, Peters W. The antimalarial activity of N-benzyloxydihydrotriazines. Ann Trop Med Parasitol 1980; 74(4): 393-404. doi: 10.1080/00034983.1980.11687360 PMID: 7006531
  17. Makler MT, Hinrichs DJ. Measurement of the lactate dehydrogenase activity of Plasmodium falciparum as an assessment of parasitemia. Am J Trop Med Hyg 1993; 48(2): 205-10. doi: 10.4269/ajtmh.1993.48.205 PMID: 8447524
  18. Uchida NS, Silva-Filho SE, Cardia GFE, et al. Hepatoprotective effect of citral on acetaminophen-induced liver toxicity in mice. Evid Based Complement Alternat Med 2017; 2017: 1-9. doi: 10.1155/2017/1796209 PMID: 28717379
  19. Hajialiani F, Elmi T, Mohamadi M, et al. Analysis of the active fraction of Iranian Naja naja oxiana snake venom on the metabolite profiles of the malaria parasite by 1HNMR in vitro. Iran J Basic Med Sci 2020; 23(4): 534-43. doi: 10.22038/IJBMS.2020.39386.9344 PMID: 32489569
  20. Hajialiani F, Shahbazzadeh D, Maleki F, Elmi T, Tabatabaie F, Zamani Z. the metabolomic profiles of sera of mice infected with plasmodium berghei and treated by effective fraction of naja naja oxiana using 1H nuclear magnetic resonance spectroscopy. Acta Parasitol 2021; 66(4): 1517-27. doi: 10.1007/s11686-021-00456-7 PMID: 34357584
  21. Neto Z, Machado M, Lindeza A, do Rosário V, Gazarini ML, Lopes D. Treatment of plasmodium chabaudi parasites with curcumin in combination with antimalarial drugs: drug interactions and implications on the ubiquitin/proteasome system. J Parasitol Res 2013; 2013(6): 1-11. doi: 10.1155/2013/429736 PMID: 23691276
  22. Elmi T, Hajialiani F, Asadi MR, et al. Antimalarial effects of the hydroalcoholic extract of Allium paradoxum in vitro and in vivo. J Parasit Dis 2021; 45(4): 1055-64. doi: 10.1007/s12639-021-01359-0 PMID: 34789989
  23. Wang H, Li Q, Reyes S, et al. Nanoparticle formulations of decoquinate increase antimalarial efficacy against liver stage Plasmodium infections in mice. Nanomedicine 2014; 10(1): 57-65. doi: 10.1016/j.nano.2013.07.010 PMID: 25177920
  24. Tripathy S, Mahapatra SK, Chattopadhyay S, et al. A novel chitosan based antimalarial drug delivery against Plasmodium berghei infection. Acta Trop 2013; 128(3): 494-503. doi: 10.1016/j.actatropica.2013.07.011 PMID: 23906613

Қосымша файлдар

Қосымша файлдар
Әрекет
1. JATS XML
Жүктеу

© Bentham Science Publishers, 2024