Exploring the Role of Cathelicidin Antimicrobial Peptide, Toll-Like Receptor 4, and HMGB-1 in Bacterial Infection
- Autores: Febriza A.1,2, Idrus H.3, Kasim V.4
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Afiliações:
- Department of Physiology, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Makassar, Makassar, Indonesia
- Postdoctoral at Centre for Biomedical Research, Research Organization for Health, National Research and Innovation Agency (BRIN), Cibinong Science Centre, Bogor, West Java, Indonesia
- Centre for Biomedical Research, Research Organization for Health, National Research and Innovation Agency (BRIN), Cibinong Science Centre, Bogor, West Java, Indonesia
- Department of Nutrition, Faculty of Medicine, State University of Gorontalo, Gorontalo, Indonesia
- Edição: Volume 22, Nº 3 (2024)
- Seção: Medicine
- URL: https://gynecology.orscience.ru/2211-3525/article/view/642316
- DOI: https://doi.org/10.2174/0122113525284634231222071749
- ID: 642316
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Resumo
method:This study is an experiment that uses a pre-post-test design. Mice balb/c were separated into three groups; group A received levofloxacin for five days, group B received a placebo, and group C was the control. Both groups, A and B, received an injection of S. Typhi strain thy1. Blood samples were taken from three groups on the 4th, 10th, and 30th day to calculate CAMP, TLR-4, and HMGB-1 mRNA gene expression levels. To determine bacterial colony, peritoneal fluid was taken three times on the 4th, 10th, and 30th day to calculate bacterial colony.
result:The expression of mRNA CAMP and bacterial colony count correlated negatively. The expression of HMGB-1 mRNA correlated with bacterial growth. Higher CAMP mRNA expression was found to relate to reduced bacterial colony count in groups A and B using linear regression.
Background and Aim:Lipopolysaccharides (LPS) from Salmonella typhi will attach with Toll-Like Receptor 4 (TLR-4) and trigger an inflammatory response to fight the pathogen. Due to infection, the HMGB1 is produced by immune cells or secreted passively from dead cells. Fur-thermore, the antimicrobial peptide, cathelicidin was secreted to neutralize and eliminate these path-ogens. This study aims to examine the interaction of Cathelicidin antimicrobial peptide (CAMP), TLR-4, and HMGB-1 on inhibiting bacterial growth in Salmonella infection.
Methods:This study is an experiment that uses a pre-post-test design. Mice balb/c were separated into three groups; group A received levofloxacin for five days, group B received a placebo, and group C was the control. Both groups, A and B, received an injection of S. Typhi strain thy1. Blood samples were taken from three groups on the 4th, 10th, and 30th day to calculate CAMP, TLR-4, and HMGB-1 mRNA gene expression levels. To determine bacterial colony, peritoneal fluid was taken three times on the 4th, 10th, and 30th day to calculate bacterial colony.
Results:Our finding observed that the expression of mRNA CAMP was inversely related to bacte-rial colony count, which means that higher CAMP mRNA expression was associated with reduced bacterial colony count in groups A and B. The expression of HMGB-1 mRNA was found to be positively correlated with bacterial growth in group A. Meanwhile, TLR-4 mRNA expression did not significantly correlate with bacterial colony count in any groups.
Conclusions:CAMP, TLR-4, and HMGB-1 affect bacterial infections. Higher expression CAMP mRNA levels lower colony counts. Meanwhile, decreasing TLR-4 and HMGB-1 mRNA expression were found during the study, due to reducing growth bacteria.
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Sobre autores
Ami Febriza
Department of Physiology, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Makassar, Makassar,Indonesia; Postdoctoral at Centre for Biomedical Research, Research Organization for Health, National Research and
Innovation Agency (BRIN), Cibinong Science Centre, Bogor, West Java, Indonesia
Autor responsável pela correspondência
Email: info@benthamscience.net
Hasta Idrus
Centre for Biomedical Research,Research Organization for Health, National Research and Innovation Agency (BRIN), Cibinong Science Centre, Bogor,
West Java, Indonesia
Email: info@benthamscience.net
Vivien Kasim
Department of Nutrition, Faculty of Medicine, State University of Gorontalo, Gorontalo, Indonesia
Email: info@benthamscience.net
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