Enviar artigo por via de e-mail Biogenic Silver Nanoparticles (AgNPs) Ameliorates Oxidative Biomarkers in Type-2 Diabetic Rats: In vitro and In vivo Report
- Autores: Akoji A.1, Ayeni G.1, Rotimi L.2, Abdulrazaq Y.2, Titus E.3, Ajuma S.1, Jamila A.1, Abayomi A.4, Christian E.1, Agah O.1, Emmanuel O.5, Faruk A.1, Moradeke O.1, Sylvester M.1, Zuleihat O.1, Dauda W.1
-
Afiliações:
- Department of Biochemistry, Prince Abubakar Audu University
- Department of Pure and Industrial Chemistry, Prince Abubakar Audu University
- Department of Medical Biochemistry, Faculty of Basic Medical Science, College of Health Sciences, Prince Abubakar Audu University
- Department of Anatomy, Faculty of Basic Medical Science, College of Health Sciences, Prince Abubakar Audu University
- Department of Biochemistry and Molecular Biology, Federal University
- Edição: Volume 18, Nº 2 (2024)
- Páginas: 71-83
- Seção: Biochemistry
- URL: https://gynecology.orscience.ru/2212-7968/article/view/643881
- DOI: https://doi.org/10.2174/0122127968284847240920103341
- ID: 643881
Citar
Texto integral
Resumo
Objective:Nanoparticles (NPs) are reliable biological tools for curative purposes through their application in nanomedicine. The present study synthesized and characterized silver nanoparticles (AgNPs) from Tetrapetra tetrapleura fruit. The investigation aims to examine the antidiabetic effect of the AgNPs using in vitro and in vivo models.
Methods:Briefly, the synthesized AgNPs were confirmed by the application of ultraviolet-visible (UV-Vis) spectroscopy, and five other techniques, viz; transmission electron microscopy (TEM) techniques, Fourier transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), X-ray diffraction analysis (XRD) and scanning electron microscope (SEM). The in vitro model assay investigated the scavenging effect of AgNPS on 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion (O2ˉ), hydroxyl anion (-OH), ferric reducing antioxidant power (FRAP), and α-amylase/α-glucosidase inhibitory activity. The in vivo model involving rats-induced type-2 diabetes with streptozotocin (STZ) was divided into six (6) groups of seven (7) rats each to assess antioxidative parameters.
Results:The AgNPs scavenged free radicals (DPPH) and moderately inhibited (O2ˉ), hydroxyl anion (-OH), reduced ferric to ferrous ions, and inhibited both α-amylase and α-glucosidase activity with increasing concentrations. Similarly, AgNPs ameliorated oxidative stress imposed by type 2 diabetes on the rats tissues significantly (p < 0.05), depleting total cholesterol, low-density lipoprotein (LDL), and increased total protein composite and high-density lipoprotein (HDL) contents. The AgNPs enhanced catalase and superoxide dismutase, reduced glutathione (GSH), and, concomitantly, decreased malondialdehyde (MDA) levels in the tissue homogenate.
Conclusion:These findings provide scientific evidence for the first time, finding the application of a biogenic compound synthesized from T. tetrapleura fruit in the treatment of type 2 diabetes.
Palavras-chave
Sobre autores
Achimi Akoji
Department of Biochemistry, Prince Abubakar Audu University
Email: info@benthamscience.net
Gideon Ayeni
Department of Biochemistry, Prince Abubakar Audu University
Autor responsável pela correspondência
Email: info@benthamscience.net
Larayetan Rotimi
Department of Pure and Industrial Chemistry, Prince Abubakar Audu University
Email: info@benthamscience.net
Yahaya Abdulrazaq
Department of Pure and Industrial Chemistry, Prince Abubakar Audu University
Email: info@benthamscience.net
Emmanuel Titus
Department of Medical Biochemistry, Faculty of Basic Medical Science, College of Health Sciences, Prince Abubakar Audu University
Email: info@benthamscience.net
Sule Ajuma
Department of Biochemistry, Prince Abubakar Audu University
Email: info@benthamscience.net
Audu Jamila
Department of Biochemistry, Prince Abubakar Audu University
Email: info@benthamscience.net
Ajayi Abayomi
Department of Anatomy, Faculty of Basic Medical Science, College of Health Sciences, Prince Abubakar Audu University
Email: info@benthamscience.net
Egwumah Christian
Department of Biochemistry, Prince Abubakar Audu University
Email: info@benthamscience.net
Ogohi Agah
Department of Biochemistry, Prince Abubakar Audu University
Email: info@benthamscience.net
Ogundipe Emmanuel
Department of Biochemistry and Molecular Biology, Federal University
Email: info@benthamscience.net
Audu Faruk
Department of Biochemistry, Prince Abubakar Audu University
Email: info@benthamscience.net
Orogun Moradeke
Department of Biochemistry, Prince Abubakar Audu University
Email: info@benthamscience.net
Monday Sylvester
Department of Biochemistry, Prince Abubakar Audu University
Email: info@benthamscience.net
Ogejah Zuleihat
Department of Biochemistry, Prince Abubakar Audu University
Email: info@benthamscience.net
Wulapba Dauda
Department of Biochemistry, Prince Abubakar Audu University
Email: info@benthamscience.net
Arquivos suplementares
